BackgroundContractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force. During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly.
DescriptionMYL2 Polyclonal Antibody, Biotin Conjugated. Biotin. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDP10916
Purification>95%, Protein G purified
ImmunogenRecombinant Human Myosin regulatory light chain 2, ventricular/cardiac muscle isoform protein (8-164AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesMyosin regulatory light chain 2, ventricular/cardiac muscle isoform, MLC-2, Cardiac myosin light chain 21, MYL2
ApplicationELISA; Recommended dilution: ELISA 1:500-1:1000