lasA Polyclonal Antibody, Biotin Conjugated

Background
Involved in proteolysis and elastolysis (degradation of the host protein elastin). Has staphylolytic activity (degrades pentaglycine cross-links in cell wall peptidogylcan), preferring Gly-Gly-/-X substrates where X is Ala or Gly (PubMed:11179971). Enhances the elastolytic but not proteolytic activity of elastase (lasB) and elastolytic activity of other proteases (PubMed:2110137). Degradation of host elastin is likely to contribute to the pathogenicity of P. aeruginosa. While either His-317 or His-356 can abstract a proton in the hydrolysis reaction, the same residue performs both functions in a given catalytic cycle, with the other stabilizing the catalytic intermediate (PubMed:20026068).

Description
lasA Polyclonal Antibody, Biotin Conjugated. Biotin. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Pseudomonas aeruginosa

Isotype
IgG

Uniprot ID
P14789

Purification
>95%, Protein G purified

Immunogen
Recombinant Pseudomonas aeruginosa Protease LasA protein (237-418AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Protease LasA, Staphylolytic protease, lasA, PA1871

Application
ELISA; Recommended dilution: ELISA 1:500-1:1000