JAK2 Recombinant Monoclonal Antibody [10B6]

Background
This gene codes for a non-receptor tyrosine kinase crucial in cytokine and growth factor signaling. The primary isoform holds an N-terminal FERM domain for erythropoietin receptor interaction, an SH2 domain for STAT transcription factor binding, a pseudokinase domain, and a C-terminal tyrosine kinase domain. Cytokine binding triggers autophosphorylation, activating the kinase. This enzyme recruits and phosphorylates STAT proteins, while growth factors like TGF-beta 1 also activate it, relocating downstream STAT proteins to the nucleus to impact gene transcription. Mutations in this gene link to inflammatory diseases and malignancies. It's a target of the IL6/JAK2/STAT3 pathway, playing a role in cellular proliferation and myeloproliferative neoplasms. A mutation in its pseudokinase domain leads to constitutive activity and cytokine hypersensitivity. This gene and the IL6/JAK2/STAT3 pathway are therapeutic targets for managing excessive inflammatory responses. Multiple transcript variants exist due to alternative splicing.

Description
JAK2 Recombinant Monoclonal Antibody [10B6]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
O60674

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human JAK2

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Tyrosine-protein kinase JAK2 (EC 2.7.10.2) (Janus kinase 2) (JAK-2), JAK2

Application
ELISA, IF; Recommended dilution: IF:1:20-1:200