EpiNext NGS Barcode (Index) Set

The EpiNext™ NGS Barcode (Index) Set is designed to construct multiplex DNA/RNA libraries used for next-generation sequencing with the Illumina platform, including GAIIx, HiSeq, MiSeq, and NextSeq. Depending on size, this product includes either 12 or 24 DNA barcodes, each containing an index and flow cell binding sequence and can be attached to the sample insert during library preparation. Multiplexing several samples in a single flow cell can be achieved by pooling with the EpiNext™ NGS Barcodes. Pooling barcoded samples into a single flow cell significantly reduces hands-on time and provides robust data quality in NGS. The EpiNext™ NGS Barcode (Index) Set has the following features:

• The barcodes contained in the EpiNext™ NGS Barcode (Index) Set can be used with single, paired-end, and multiplex reads and are compatible with various library preparation workflows used for Illumina NGS, including DNA-seq, RNA-seq, ChIP-seq, MethylC-seq, MeDIP-seq, hMeDIP-seq, DNA bisulfite-seq, oxBS-seq, and RNA bisulfite-seq. 
• The barcodes are also optimized for use with EpigenTek’s EpiNext™ NGS library preparation kit series including EpiNext™ DNA Library Preparation Kit (Illumina), EpiNext™ High-Sensitivity DNA Library Preparation Kit (Illumina), EpiNext™ Post-Bisulfite DNA Library Preparation Kit (Illumina), EpiNext™ Bisulfite-Seq High-Sensitivity Kit (Illumina), EpiNext™ ChIP-Seq High-Sensitivity Kit (Illumina), EpiNext™ RNA Bisulfite-Seq Kit and EpiNext™ MeDIP-Seq Kit.
• High throughput and cost reduction: Up to 144 or 288 multiplexed samples can be processed, which allows multiplexing of 2-12 or 2-24 fragment library samples on a single sequencing flow cell resulting in considerable reduction of per-sample cost.
• The indices are designed to be error-resistant and allow for proper differentiation between samples.
• Minimizes PCR amplification-based bias and prevents poor reads from single base errors introduced during PCR.