Recombinant SARS-CoV-2 Spike S1 Protein with His-Tag

Background
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Description
Recombinant SARS-CoV-2 Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val16-Arg685) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6xHis tag at the C-terminus.

Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike S1 at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL. Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 11.4 nM as determined in an SPR assay (Nicoya OpenSPR).

Purity
>90% by SDS-PAGE;> 95% by HPLC

Endotoxin
< 1.0 EU/ug of the protein by LAL method.

Formulation
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4. or Supplied as a 0.22 um filtered solution in PBS, pH 7.4.

Species
SARS-COV-2

Calculated MW
75.8kDa

Expressed Host
HEK293 cells

Tag
6xHis tag at the C-terminus

Gene ID
43740568

Storage
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at the recommended temperature.

Reconstitution
For lyophilized protein: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Alternative Names
S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; novel coronavirus s1 Protein; SARS-CoV-2