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Histone H3K9ac (Acetyl H3K9) Polyclonal Antibody

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Application: ChIP, ChIP-Seq, ELISA, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-4022-02525 µg $139.00 
A-4022-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background
Histone H3- along with H2A, H2B and H4- is involved in the structure of chromatin in eukaryotic cells. Histone H3 can undergo several different types of epigenetic modifications that influence cellular processes. These modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occur on the N-terminal tail domains of histone H3, which results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23.

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of histone H3 acetylated on K9, ChIP-grade

Purification
Protein A purified

Specificity
Detects histone H3 only when acetylated at K9 in mouse, rat, and human

Isotype
IgG

Formulation 
10 mM Tris (pH 7.5), 150 mM NaCl, and 50% glycerol

Storage
-20°C, stable for 1 year from the date of shipment. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance

Alternative Names
H3K9ac antibody, H3K9a antibody

Application 
WB: 1:200-1:1000, IHC: 1:100-1:500, ELISA: 1:1000-1:2000, IP: 2 μg/106 cells

Fig. 1. WB analysis of Histone H3K9ac (Acetyl H3K9) Polyclonal Antibody with HeLa cell lysates (A-4022).

User Guide & MSDS

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