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Histone H3K18ac (Acetyl H3K18) Polyclonal Antibody

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Application: ChIP, ChIP-Seq, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-4024-02525 µg $139.00 
A-4024-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of histone H3 acetylated on K18, ChIP-grade

Purification
Affinity purified

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Immunogen 
A synthetic acetylated peptide corresponding to residues surrounding K18 of human H3

Formulation 
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Storage
Store at -20°C or -80°C. Avoid freeze / thaw cycles.

Alternative Names
H3K18ac antibody; H3K18a antibody;H3t; H3.4; H3/g; H3FT

Application 
WB 1:1000 - 1:3000
IHC 1:200- 1:500
IF 1:500- 1:1000
IP 1:200- 1:500
ChIP 1:50- 1:100
ChIP-Seq 1:50- 1:100

Fig. 1. Western blot analysis of extracts of various cell lines, using Histone H3K18ac (Acetyl H3K18) Polyclonal Antibody.

 

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