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EpiQuik Global Histone H3K4 Methylation Assay Kit

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Schematic procedure for using the EpiQuik Global Histone H3K4 Methylation Assay Kit.
Input Type: Cells, Unisolated Samples
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3017-4848 assays $369.00 
P-3017-9696 assays $549.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Global Histone H3-K4 Methylation Assay Kit is suitable for specifically measuring global histone H3-K4 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global H3-K4 Methylation Assay Kit is designed for measuring global histone H3-K4 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K4 can be recognized with a high-affinity antibody The ratio or amount of methylated H3-K4 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

Product Components

GD1 (10X Lysis Buffer)
GD2 (Extraction Buffer)
GD3 (10X Wash Buffer)
GD4 (Histone Buffer)
GD5 (Blocking Buffer)
GD6 (Antibody Buffer)
GD7 (Capture Antibody, 100 µg/ml)*
GD8 (Detection Antibody, 400 µg/ml)*
GD9 (Developing Solution)
GD10 (Stop Solution)
Methylated H3-K4 Control (60 µg/ml)*
8-Well Assay Strips (with frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Ding S et. al. (November 2017). Chronic sun exposure is associated with distinct histone acetylation changes in human skin. Br J Dermatol.

Bilan Li et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation Journal of Cancer. 8:933 -939.

Li B et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation. J Cancer. 8(6):933-939.

Zhou K et. al. (February 2017). Aberrant histone modification in CD19<sup>+</sup> B cells of patients with chronic lymphocytic leukemia. Onco Targets Ther. 10:1173-1179.

Yang Y et. al. (March 2015). The effect of mycophenolic acid on epigenetic modifications in lupus CD4<sup>+</sup>T cells. Clin Immunol.

Zhao H et. al. (January 2014). Aberrant histone methylation in the patients with immune thrombocytopenia. Platelets. 25(3):207-10.

Xiaomeng X et. al. (March 2013). Aberrant histone acetylation and methylation levels in woman with endometriosis. Arch Gynecol Obstet. 287(3):487-94.

Batra V et. al. (March 2012). Interaction between γ-radiation and dietary folate starvation metabolically reprograms global hepatic histone H3 methylation at lysine 4 and lysine 27 residues. Food Chem Toxicol. 50(3-4):464-72.

Zhao M et. al. (February 2012). Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata. Br J Dermatol. 166(2):226-73.

Chen C et. al. (October 2011). Abnormal histone acetylation and methylation levels in esophageal squamous cell carcinomas. Cancer Invest. 29(8):548-56.

Hu N et. al. (May 2008). Abnormal histone modification patterns in lupus CD4+ T cells. J Rheumatol. 35(5):804-10.

Customer Reviews

Rating via Distributor/Reseller Verified Purchase Reviewed on: Tuesday 21 November, 2017
Application Description
We analyzed the histone modifications associated with biologic drugs in different time points of the treatment following the manufacturers┬┤ instructions. We also analyzed the differences in histone methylation between patients from the dermatology unit and healthy subjects. It is ready to use, easy and fast to perform.

Other Thoughts
The amount of Antibody buffer (GD6) is quite restrictive. The kit does not include enough volume to perform pre-dilutions of the samples. Moreover, the strip wells do not bind properly to the plate. Therefore, it sometimes causes troubles when you introduce the plate in the Multiskan, causing it to get stuck. When we measured the background of the empty plates that had been at room temperature for at least 15 minutes, we saw that the background was not very homogenous. As the expected differences between our case and control samples were quite small, the variability found in the background may mask the specific differences within them. However, I think there must be difficult to reduce such variation. It is important to underlie that all materials needed are included in the kit. To carry out the experiments you only need a microplate spectrophotometer and a centrifuge.


Differences in the percentage of methylated H3K4 in peripheral blood mononuclear cells.


Differences in the percentage of methylated H3K4 in peripheral blood mononuclear cells between our cases and controls.
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