Product Overview
The EpiQuik™ Global Histone H3-K4 Methylation Assay Kit is suitable for specifically measuring global histone H3-K4 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
WHY CHOOSE THE EPIQUIK™ GLOBAL HISTONE H3-K4 METHYLATION ASSAY KIT?
- Quick and efficient procedure, which can be finished within 5 hours.
- Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
- Strip microwell format makes the assay flexible: manual or high throughput.
- Simple, reliable, and consistent assay conditions.
Product Details
The EpiQuik™ Global H3-K4 Methylation Assay Kit is designed for measuring global histone H3-K4 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K4 can be recognized with a high-affinity antibody The ratio or amount of methylated H3-K4 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
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Product Components
GD1 (10X Lysis Buffer)
GD2 (Extraction Buffer)
GD3 (10X Wash Buffer)
GD4 (Histone Buffer)
GD5 (Blocking Buffer)
GD6 (Antibody Buffer)
GD7 (Capture Antibody, 100 µg/ml)*
GD8 (Detection Antibody, 400 µg/ml)*
GD9 (Developing Solution)
GD10 (Stop Solution)
Methylated H3-K4 Control (60 µg/ml)*
8-Well Assay Strips (with frame)
User Guide
* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.
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