The EpiQuik™ Global Histone H3-K27 Methylation Assay Kit is suitable for specifically measuring global histone H3-K27 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL HISTONE H3-K27 METHYLATION ASSAY KIT?

• Quick and efficient procedure, which can be finished within 5 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Global H3-K27 Methylation Assay Kit is designed for measuring global histone H3-K27 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K27 can be recognized with a high-affinity antibody. The ratio or amount of methylated H3-K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:

GG1 (10x lysis buffer)
GG2 (Extraction buffer)
GG3 (10X Wash Buffer)
GG4 (Histone Buffer)
GG5 (Blocking Buffer)
GG6 (Antibody Buffer)
GG7 (Capture Antibody, 100 µg/ml)*
GG8 (Detection Antibody, 400 µg/ml)*
GG9 (Developing Solution)
GG10 (Stop solution)
Methylated H3-K27 Control (60 µg/ml)*
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Chen, C. et. al. (October 2011). Abnormal histone acetylation and methylation levels in esophageal squamous cell carcinomas. Cancer Invest. 29(8):548-56. PubMed Abstract