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Histone H3K79me2 (H3K79 Dimethyl) Polyclonal Antibody

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Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K79me2 Polyclonal Antibody.
Dot-blot analysis of all sorts of methylation peptides using H3K79me2 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded rat kidney tissue using H3K79me2 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded human kidney cancer tissue using H3K79me2 Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunofluorescence analysis of 293T cell using H3K79me2 Polyclonal Antibody. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K79 Polyclonal Antibody and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application: ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Guarantee: 6 months
Catalog No.SizePriceQty
A-4044-02525 µg $139.00 
A-4044-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background 
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome.

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody to the dimethylated histone H3 at K79, ChIP-grade

Immunogen 
A synthetic peptide of human Histone H3K79me2

Purification
Affinity purified

Specificity
Human, mouse, rat and broad range

Isotype
IgG

Formulation 
PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Storage
Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles.

Alternative Names
H3.4; H3/g; H3FT; H3t; MGC126886; MGC126888; H3K79me2 antibody; H3K79m2 antibody

Application 
WB: 1:500 - 1:1000, IHC: 1:50 - 1:100, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:50 - 1:200, ChIP-seq: 1:50-1:200

Fig. 1. Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K79me2 Polyclonal Antibody.

Fig. 2. Dot-blot analysis of all sorts of methylation peptides using H3K79me2 Polyclonal Antibody.

Fig. 3. Immunohistochemistry of paraffin-embedded rat kidney tissue using H3K79me2 Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 4. Immunohistochemistry of paraffin-embedded human kidney cancer tissue using H3K79me2 Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 5. Immunofluorescence analysis of 293T cell using H3K79me2 Polyclonal Antibody. Blue: DAPI for nuclear staining.

Fig. 6. Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K79 Polyclonal Antibody and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

User Guide & MSDS

[User Guide]*
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Product Citations

Ma P et. al. (February 2012). Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A. 109(8):E481-9.

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