p300, also known as EP300 or E1A binding protein p300, is a universally expressed, reasonably stable nuclear phosphorprotein that is closely related to CBP (or CREB). CBP and p300 are identified as transcriptional coactivators that can directly interact with several different DNA-binding transcription factors including nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. These two proteins have been known to exhibit histone acetyltransferase (HAT) activity and can acetylate all four core histones in the nucleosome. It is believed that both p300 and CBP may be directly involved in activating chromatin for transcription.
Rabbit polyclonal antibody to p300, ChIP-grade
Protein A purified
Mouse, rat, human
0.1% Sodium Azide, PBS
4ºC, stable for 1 year from the date of shipment. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance
CREBBP/EP300 inhibitory protein 1 antibody, P-300, Cyclic AMP responsive enhancer binding protein antibody, E1A associated protein p300 antibody, E1A binding protein p300 antibody, EC 188.8.131.52 antibody, EP300 antibody, Histone acetyltransferase p300 antibody, p300 HAT antibody, RB and P300 binding protein EID 1 antibody, Retinoblastoma protein associated protein antibody, RSTS2 antibody
WB: 1:2000-1:10000, IF: 1:500-1:5000, IHC: 1:500-1:5000, ELISA: 1:300-1:30000, IP: 0.2 μg/10^6 cells
Fig. 1. WB analysis of p300 Polyclonal Antibody with KNRK cell lysates (A-4020).