Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy
Rabbit polyclonal to the monomethylated histone H4 at K20, ChIP-grade
A synthetic peptide corresponding to the amino terminus of histone H4 in which Lys20 is mono-methylated.
Human, mouse, rat and broad range
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Store at -20ºC (regular) or -80ºC (long term). Avoid freeze / thaw cycles.
H4; H4/n; H4F2; H4FN; FO108; HIST2H4; H4K20me1 antibody; H4K20m1 antibody
WB: 1:500 - 1:2000, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:50 - 1:200
Fig. 1. Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4K20me1 antibody.
Fig. 2. Dot-blot analysis of all sorts of methylation peptides using H4K20me1 antibody.
Fig. 3. Immunofluorescence analysis of 293T cell using H4K20me1 antibody. Blue: DAPI for nuclear staining.