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Histone H4K20me1 (H4K20 Monomethyl) Polyclonal Antibody

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Application: ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-4046-02525 µg $139.00 
A-4046-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background 
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy

Concentration
1 mg/ml

Description
Rabbit polyclonal to the monomethylated histone H4 at K20, ChIP-grade

Purification
Affinity purified

Immunogen
A synthetic peptide corresponding to the amino terminus of histone H4 in which Lys20 is mono-methylated.

Specificity
Human, mouse, rat and broad range

Isotype
IgG

Formulation 
PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Storage
Store at -20ºC (regular) or -80ºC (long term). Avoid freeze / thaw cycles.

Alternative Names
H4; H4/n; H4F2; H4FN; FO108; HIST2H4; H4K20me1 antibody; H4K20m1 antibody

Application 
WB: 1:500 - 1:2000, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:50 - 1:200

Fig. 1. Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4K20me1 antibody.

Fig. 2. Dot-blot analysis of all sorts of methylation peptides using H4K20me1 antibody.

Fig. 3. Immunofluorescence analysis of 293T cell using H4K20me1 antibody. Blue: DAPI for nuclear staining.

User Guide & MSDS

[User Guide]*
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