Epigentek Home
 
Epigentek Free Shipping

Histone H3K9me2 (H3K9 Dimethyl) Polyclonal Antibody

Share:

Citations (6) | Write a Review
Application: ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-4035-02525 µg $139.00 
A-4035-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background 
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the monomethylated histone H3 at K9, ChIP-grade

Purification
Affinity purified

Specificity
Human, Mouse, Rat, Broad Range

Isotype
IgG

Formulation 
Serum comprising 0.03% sodium azide, 30% glycerol

Storage
Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Alternative Names
H3K9me2 antibody, H3K9m2 antibody; Anti-Histone H3K9;  HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3;

Application 
WB: 1:500 - 1:2000, IHC:1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:50 - 1:200

Fig. 6. Immunohistochemistry of paraffin-embedded rat testis tissue using H3K9me2 antibody at dilution of 1:200 (x400 lens).

Fig. 7. Immunohistochemistry of paraffin-embedded rat brain tissue using H3K9me2 antibody at dilution of 1:200 (x400 lens). 

Fig. 8. Immunohistochemistry of paraffin-embedded human thyroid cancer tissue using H3K9me2 antibody at dilution of 1:200 (x400 lens).

Fig. 9. Immunohistochemistry of paraffin-embedded rat liver tissue using H3K9me2 antibody at dilution of 1:200 (x400 lens).

Fig. 10. Immunohistochemistry of paraffin-embedded mouse kidney tissue using H3K9me2 antibody at dilution of 1:200 (x400 lens).

Fig. 11. Immunohistochemistry of paraffin-embedded rat lung tissue using H3K9me2 antibody at dilution of 1:200 (x400 lens).


Fig. 1. Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K9me2 antibody.


Fig. 2. Dot-blot analysis of all sorts of methylation peptides using H3K9me2 antibody.

 

Fig. 3. Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethyl-Histone H3-K9 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Fig. 4. Immunofluorescence analysis of 293T cell using H3K9me1 antibody. Blue: DAPI for nuclear staining.

Fig. 5. Immunohistochemistry of paraffin-embedded human rectal cancer tissue using H3K9me2 antibody at dilution of 1:200 (x400 lens).

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2016 Epigentek Group Inc. All rights reserved.