Concentration
1 mg/ml

Description
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the dimethylated histone H3 at K9, ChIP-grade

Specificity
Detects histone H3 only when dimethylated at K9 in mouse, rat, and human. Broad species to cross-reactivity is also expected.

Isotype
IgG

Formulation
PBS (pH 7.5), 150 mM NaCl, 30% glycerol

Storage
-20°C, stable for 1 year from the date of shipment. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Application
WB: 1:1000-1:2000, IF: 1:100-1:500, IH: 1:100-1:500, ELISA: 1:1000-1:2000, IP: 2 μg/10⁶ cells

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Hirai, S. et. al. (April 2010). Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco. FEBS Journal. 277(7): 1695-703. PubMed Abstract