The EpiQuik™ HDAC8 Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of HDAC8 at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful HDAC8 assay experiment without the need for electrophoresis and transfer process. It is suitable for measuring HDAC8 level from various fresh tissues and cultured mammalian cells.

WHY CHOOSE THE EPIQUIK™ HDAC8 ASSAY KIT?

• Fast procedure, which can be finished within 3 hours.
• Innovative colorimetric assay to semi-quantitatively measure HDAC8 amount without the need for electrophoresis.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ HDAC8 Assay Kit is designed for measuring total HDAC8 amount from tissues or cells. In an assay with this kit, the nuclear proteins containing HDAC8 are stably coated on the strip wells. The HDAC8 is recognized with a high-affinity specific antibody. The amount of HDAC8 can be quantified through a HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:

HC1 (10X Wash Buffer)
HC2 (HDAC Assay Buffer)
HC3 (Blocking Buffer)
HC4 (Capture Antibody, 200 µg/ml)*
HC5 (Detection Antibody, 200 µg/ml)*
HC6 (Developing Solution)
HC7 (Stop Solution)
HDAC8 Control (100 ng/µl)
8-Well Assay Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]