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Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody


Citations (8) | Write a Review
Application: ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-4036-02525 µg $139.00 
A-4036-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

1 mg/ml

Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the trimethylated histone H3 at K9, ChIP-grade

Affinity purification

Broad Range, Mouse, Rat, Human


A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles.

Alternative Names
H3K9me3 antibody; H3K9m3 antibody; HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3 

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIP: 1:50 - 1:200
ChIP-Seq: 1:50 - 1:200

Fig. 1. Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K9me3 Polyclonal Antibody.

Fig. 2. Dot-blot analysis of all sorts of methylation peptides using H3K9me3 Polyclonal Antibody.

Fig. 3. Immunohistochemistry of paraffin-embedded rat lung tissue using H3K9me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 4. Immunohistochemistry of paraffin-embedded human kidney tissue using H3K9me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 5. Immunohistochemistry of paraffin-embedded human lung cancer tissue using H3K9me3 antibody at dilution of 1:200 (x400 lens).

Fig. 6. Immunohistochemistry of paraffin-embedded human liver cancer tissue using H3K9me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 7. Immunofluorescence analysis of 293T cell using H3K9me3 Polyclonal Antibody. Blue: DAPI for nuclear staining.

Fig. 8. Chromatin immunoprecipitation analysis extracts of 293 cell line, using Histone H3K9 Trimethyl Polyclonal Antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

User Guide & MSDS

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Product Citations

Jiajia Xu et. al. (September 2016). Expression of SIRT1, H3K9me3, H3K9Ac and E-cadherin and its correlations with clinicopathological characteristics in gastric cancer patients IJCEP. 9(9)

Timoshevskiy VA et. al. (June 2016). Cellular and Molecular Features of Developmentally Programmed Genome Rearrangement in a Vertebrate (Sea Lamprey: Petromyzon marinus). PLoS Genet. 12(6):e1006103.

Trapphoff T et. al. (November 2015). Postovulatory aging affects dynamics of mRNA, expression and localization of maternal effect proteins, spindle integrity and pericentromeric proteins in mouse oocytes. Hum Reprod.

Tyler CR et. al. (July 2015). Developmental exposure to 50 parts-per-billion arsenic influences histone modifications and associated epigenetic machinery in a region- and sex-specific manner in the adult mouse brain. Toxicol Appl Pharmacol.

Wiegreffe C et. al. (July 2015). Bcl11a (Ctip1) Controls Migration of Cortical Projection Neurons through Regulation of Sema3c. Neuron. 87(2):311-25.

Kumar P et. al. (March 2014). Histone deacetylase inhibitors modulate the transcriptional regulation of guanylyl cyclase/natriuretic peptide receptor-a gene: interactive roles of modified histones, histone acetyltransferase, p300, AND Sp1. J Biol Chem. 289(10):6991-7002.

Sui WG et. al. (January 2014). ChIP-seq analysis of histone H3K9 trimethylation in peripheral blood mononuclear cells of membranous nephropathy patients. Braz J Med Biol Res. 47(1):42-9.

Trapphoff T et. al. (December 2013). Chronic exposure to a low concentration of bisphenol A during follicle culture affects the epigenetic status of germinal vesicles and metaphase II oocytes. Fertil Steril. 100(6):1758-67.e1.

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