Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p, is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases. It methylates histones and MBP in vitro. Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p. An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK). Both proteins belong to the protein methyltransferase superfamily. Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases. This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells.
A synthetic peptide of human PRMT5
Rabbit polyclonal antibody to PRMT5
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles.
72 kDa ICln binding protein antibody, ANM5_HUMAN antibody, Histone synthetic lethal 7 S. cerevisiae homolog of antibody, Histone-arginine N-methyltransferase PRMT5 antibody, HMT1 hnRNP methyltransferase like 5 antibody, HRMT1L5 antibody, IBP72 antibody, Jak-binding protein 1 antibody, JBP 1 antibody, PRMT 5 antibody, Protein arginine N methyltransferase 5 N terminally processed antibody, S. Pombe antibody, Shk1 kinase binding protein 1 homolog antibody, SKB 1 antibody, SKB1Hs antibody
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:20 - 1:100
Fig. 1. Western blot analysis of extracts of various cell lines, using PRMT5 antibody.
Fig. 2. Immunohistochemistry of paraffin-embedded human stomach using PRMT5 antibody at dilution of 1:200 (x400 lens).
Fig. 3. Immunofluorescence analysis of A549 cell using PRMT5 antibody. Blue: DAPI for nuclear staining.