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PRMT5 Polyclonal Antibody

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Application: ChIP, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-3005-05050 µg $199.00 
A-3005-100100 µg $369.00 
Availability: Usually Ships in 3-4 Days 
Product Overview

Background
Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p, is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases. It methylates histones and MBP in vitro. Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p. An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK). Both proteins belong to the protein methyltransferase superfamily. Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases. This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells.

Immunogen
A synthetic peptide of human PRMT5

Description
Rabbit polyclonal antibody to PRMT5

Purification
Affinity purification

Specificity
Mouse, Human, Rat

Isotype
IgG

Concentration
1mg/ml

Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage
Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles.

Alternative Names
72 kDa ICln binding protein antibody, ANM5_HUMAN antibody, Histone synthetic lethal 7 S. cerevisiae homolog of antibody, Histone-arginine N-methyltransferase PRMT5 antibody, HMT1 hnRNP methyltransferase like 5 antibody, HRMT1L5 antibody, IBP72 antibody, Jak-binding protein 1 antibody, JBP 1 antibody, PRMT 5 antibody, Protein arginine N methyltransferase 5 N terminally processed antibody, S. Pombe antibody, Shk1 kinase binding protein 1 homolog antibody, SKB 1 antibody, SKB1Hs antibody

Application
WB: 1:500 - 1:2000, IHC: 1:50 - 1:100, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:20 - 1:100


Fig. 1. Western blot analysis of extracts of various cell lines, using PRMT5 Polyclonal Antibody.


Fig. 2. Immunohistochemistry of paraffin-embedded rat brain using PRMT5 Polyclonal Antibody at dilution of 1:100 (x40 lens).


Fig. 3. Immunohistochemistry of paraffin-embedded mouse brain using PRMT5 Polyclonal Antibody at dilution of 1:100 (x40 lens).


Fig. 4. Immunohistochemistry of paraffin-embedded human colon using PRMT5 Polyclonal Antibody at dilution of 1:100 (x40 lens).


Fig. 5. Immunohistochemistry of paraffin-embedded rat brain using PRMT5 Polyclonal Antibody at dilution of 1:100 (x40 lens).

 


Fig. 6. Immunofluorescence analysis of U2OS cell using PRMT5 Polyclonal Antibody. Blue: DAPI for nuclear staining.

 


 Fig. 7. Immunofluorescence analysis of A549 cell using PRMT5 Polyclonal Antibody. Blue: DAPI for nuclear staining.

 

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