Epigentek Home
Epigentek Free Shipping

Histone H3K9me1 (H3K9 Monomethyl) Polyclonal Antibody


Citations (1) | Write a Review
Application: ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Guarantee: 6 months
Catalog No.SizePriceQty
A-4034-02525 µg $139.00 
A-4034-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.

1 mg/ml


Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the monomethylated histone H3 at K9, ChIP-grade

Affinity purified

Human, Mouse, Rat, Broad Range


Serum comprising 0.03% sodium azide, 30% glycerol

Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Alternative Names
H3K9me1 antibody, H3K9m1 antibody;HIST1H3J; H3/j ;H3FJ; Histone H3.1; Histone H3/a; Histone H3/b;Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j;Histone H3/k; Histone H3/l; HIST3H3

WB: 1:500 - 1:2000, IHC:1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:50 - 1:200,  ChIP-seq: 1:20 - 1:100

Fig. 5. Immunohistochemistry of paraffin-embedded rat testis tissue using H3K9me1 Monomethyl Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 6. Immunohistochemistry of paraffin-embedded human embryo brain tissue using H3K9me1 Monomethyl Polyclonal Antibody  at dilution of 1:200 (x400 lens).



Fig. 1. Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K9me1 Monomethyl Polyclonal Antibody.

Fig. 2. Dot-blot analysis of all sorts of methylation peptides using H3K9me1 Monomethyl Polyclonal Antibody.

Fig. 3. Chromatin immunoprecipitation analysis extracts of 293 cell line, using MonoMethyl-Histone H3-K9 Polyclonal Antibody and rabbit IgG. The amount of immunoprecipitated DNA to the input..

Fig. 4. Immunofluorescence analysis of 293T cell using H3K9me1 Monomethyl Polyclonal Antibody . Blue: DAPI for nuclear staining.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Related Products

Researchers Also Purchased
Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody
Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody
Histone H3K9me2 (H3K9 Dimethyl) Polyclonal Antibody
Histone H3K9me2 (H3K9 Dimethyl) Polyclonal Antibody
Histone H3K27me1 (H3K27 Monomethyl) Polyclonal Antibody
Histone H3K27me1 (H3K27 Monomethyl) Polyclonal Antibody
Histone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody
Histone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2016 Epigentek Group Inc. All rights reserved.