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Histone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody

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Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K4me1 Monomethyl Polyclonal Antibody.
Dot-blot analysis of all sorts of methylation peptides using H3K4me1 Monomethyl Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded rat spleen tissue using H3K4me1 Monomethyl Polyclonal Antibody at dilution of 1:200 (x400 lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer tissue using H3K4me1 Monomethyl Polyclonal Antibody at dilution of 1:200 (x401 lens).
Immunohistochemistry of paraffin-embedded rat brain tissue using H3K4me1 Monomethyl Polyclonal Antibody at dilution of 1:200 (x402 lens).
Immunohistochemistry of paraffin-embedded rat kidney tissue using H3K4me1 Monomethyl Polyclonal Antibody at dilution of 1:200 (x403 lens).
Immunofluorescence analysis of 293T cell using H3K4me1 Monomethyl Polyclonal Antibody. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using Histone H3K4 Monomethyl Polyclonal Antibody and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked_x001F_ by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application: ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Guarantee: 6 months
Catalog No.SizePriceQty
A-4031-02525 µg $139.00 
A-4031-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the monomethyled histone H3 at K4, ChIP-grade

Purification
Affinity purification

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Immunogen
A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3

Formulation 
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Storage
Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles.

Alternative Names
H3K4me1 antibody; H3K4m1 antibody; HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/ ; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3

Application 
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
ChIP-seq 1:20 - 1:100


Fig. 1. Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K4me1 Monomethyl Polyclonal Antibody.

 
Fig. 2. Dot-blot analysis of all sorts of methylation peptides using H3K4me1 Monomethyl Polyclonal Antibody.

 
Fig. 3. Immunohistochemistry of paraffin-embedded rat spleen tissue using H3K4me1 Monomethyl Polyclonal Antibody at dilution of 1:200 (x400 lens).

 
Fig. 4. Immunohistochemistry of paraffin-embedded human thyroid cancer tissue using H3K4me1 Monomethyl Polyclonal Antibody at dilution of 1:200 (x401 lens).


Fig. 5. Immunohistochemistry of paraffin-embedded rat brain tissue using H3K4me1 Monomethyl Polyclonal Antibody at dilution of 1:200 (x402 lens).

 
Fig. 6. Immunohistochemistry of paraffin-embedded rat kidney tissue using H3K4me1 Monomethyl Polyclonal Antibody at dilution of 1:200 (x403 lens).

 
Fig. 7. Immunofluorescence analysis of 293T cell using H3K4me1 Monomethyl Polyclonal Antibody. Blue: DAPI for nuclear staining.

 
Fig. 8. Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using Histone H3K4 Monomethyl Polyclonal Antibody and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

 

User Guide & MSDS

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