O6-methylguanine methyltransferase (MGMT) is a ubiquitous DNA repair protein that removes O6 -alkyl-guanine lesions, primarily O6 -methyl-guanine from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation. Also, MGMT is frequently silenced due to abnormal methylation during cancer development. Consequently, the loss of MGMT activity that made the cell more likely to become a cancer cell in the first place also makes tumor cells more sensitive to radiation therapy and certain alkylating drugs.
Rabbit polyclonal antibody to MGMT, ChIP-grade
Protein A purified
Mouse, rat, human
0.1% Sodium Azide, 0.1% Gelatin, PBS
4°C, stable for 1 year from the date of shipment. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance
6 O methylguanine DNA methyltransferase antibody, Agat antibody, AGT antibody, AI267024 antibody, EC 220.127.116.11 antibody, Methylated DNA protein cysteine methyltransferase antibody, Methylguanine DNA methyltransferase antibody, MGC107020 antibody, MGMT antibody, O 6 methylguanine DNA alkyltransferase antibody, O 6 methylguanine DNA methyltransferase antibody
WB: 1:200-1:500; IF: 1:100-1: 500, ELISA: 1:1000-1:2000; IP: 2 µg/106 cells
Fig. 1. WB analysis of MGMT antibody with MCF-7 nuclear extracts (A-1010).