Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to N-terminus of histone H4 acetylated at K8, ChIP-grade
Broad Range, Mouse, Rat, Human
A synthetic acetylated peptide corresponding to residues surrounding K8 of human H4
PBS (pH 7.5), 0.1% gelatin
4°C, stable for 1 year from the date of shipment. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance
H4K8ac antibody; H4K8a antibody;H4; H4/n; H4F2; H4FN; FO108; HIST2H4
WB 1:1000 - 1:3000
IHC 1:200 - 1:500
IF 1:500 - 1:1000
IP 1:200 - 1:500
ChIP 1:20 - 1:50
ChIP-Seq 1:20 - 1:50
Fig. 1. Western blot analysis of extracts of 293T cell line and H4 protein expressed in E.coli, using Histone H4K8ac (Acetyl H4K8) Polyclonal Antibody.