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EpiQuik Tissue Acetyl-Histone H3 ChIP Kit

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For immunoprecipitating H3ac chromatin from tissue input samples via microplate format

Citations (9) | (1) | Write a Review
Schematic procedure of the EpiQuik™ Tissue Acetyl-Histone H3 ChIP Kit.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2012-2424 reactions $338.00 
P-2012-4848 reactions $539.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Tissue Acetyl-Histone H3 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3 acetylation and DNA in variety of mammalian tissues. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Tissue Acetyl-Histone H3 ChIP kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip. This kit has the following advantages: 

  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

Background Information
Histone acetylation takes place at the α-amino groups of conserved lysine residues, where histone acetyltransferases (HATs) are responsible for catalyzing this modification. It has been demonstrated that histone H3 acetylation serves as an epigenetic marker of chromosomal domains. Histone H3 acetylation regulates various cellular physiological processes, including transcriptional activation of genes, chromatin assembly, cell proliferation, and some pathological processes such as tumorigenesis. Chromatin Immunoprecipitation (ChIP) is a powerful technique for studying protein-DNA interaction in vivo. ChIP also offers an advantageous tool that allows identification of activated genes associated with acetylated histone H3. ChIP coupled with microarrays could be further used for profiling or mapping histone H3 acetylation patterns. 

Principle & Procedure
This ChIP kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for acetyl-histone H3 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H3 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured acetyl-histone H3 protein-DNA complex, reversed and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various down-stream applications.  

Starting Materials
Starting materials can include various tissue samples. In general, the input amount should be 20 mg of tissue for each reaction.

Product Components

CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3 (Lysis Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Homogenizing Buffer
Protease Inhibitor cocktail (100X)*
Proteinase K (10 mg/ml)*
Normal Mouse IgG (0.5 mg/ml)*
Anti-Acetyl-Histone H3 (1 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Qian L. et al. et. al. (September 2017). Exacerbation of diabetic cardiac hypertrophy in OVE26 mice by angiotensin II is associated with JNK/c-Jun/miR-221-mediated autophagy inhibition Oncotarget.

Xu Z et. al. (August 2017). Inhibition of HDAC3 prevents diabetic cardiomyopathy in OVE26 mice via epigenetic regulation of DUSP5-ERK1/2 pathway. Clin Sci (Lond). 131(15):1841-1857.

Li B et. al. (September 2016). Relationship Between the Altered Expression and Epigenetics of GSTM3 and Age-Related Cataract. Invest Ophthalmol Vis Sci. 57(11):4721-32.

Scholpa NE et. al. (January 2016). Cyclin-dependent kinase inhibitor 1a (p21) modulates response to cocaine and motivated behaviors. J Pharmacol Exp Ther.

Hervouet E et. al. (October 2015). The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models. BMC Cancer. 15(1):729.

Zhu X et. al. (October 2015). Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract. J Ophthalmol. 2015:579695.

Tao W et. al. (June 2014). Persistent inflammation-induced upregulation of BDNF promotes synaptic delivery of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor GluA1 subunits in descending pain modulatory circuits. J Biol Chem.

Shen W et. al. (June 2014). Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated fatty acids. Sci Rep. 4:5282.

Shio S et. al. (April 2011). Loss of RUNX3 expression by histone deacetylation is associated with biliary tract carcinogenesis. Cancer Sci. 102(4):776-83.

Customer Reviews

Rating by k****@uky.edu Verified Purchase Reviewed on: Wednesday 04 February, 2015
Application Description
We used this product on frozen rat brain whole tissues and got very good yield of chromatin. The protocol is simple, easy to follow and worked well. 
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