Product Overview
The EpiQuik™ In Situ DNA Damage Assay Kit is a convenient set of tools that allows the experimenter to detect DNA damage or apoptosis by measuring phosphorylation of H2AXSer139 in situ. The kit is ready-to-use and provides all the essential components needed for specifically measuring DNA damage in situ through phospho H2AXSer139 detection using cultured adherent cells.
WHY CHOOSE THE EPIQUIK™ IN SITU DNA DAMAGE ASSAY KIT?
- Quick and efficient procedure, which can be finished within 3 hours.
- Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
- Measurement of in situ histone H2AX phosphorylation without the need to prepare cell lysates.
- Microplate format makes the assay suitable for high throughput analysis of agents that increases or inhibits DNA damage.
- Simple, reliable, and consistent assay conditions.
Product Details
EpiQuik™ In Situ DNA Damage Assay Kit is a whole cell-based detection of DNA damage and/or apoptosis. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The phosphorylation of H2AX at serine139, the sensitive marker of DNA damage, is then detected by an anti-phospho H2AXSer139 antibody. The ratio or amount of phospho H2AXSer139 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
| SCHEMATIC PROCEDURE: |
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 MCF-7 cells in microplate wells were exposed to etoposide for 4 h and DNA damage was detected by the measuring level of phosphor-H2AX. |
Product Components
A1 (10X Wash Buffer)
A2 (Permeabilizing Buffer)
A3 (Blocking Buffer)
A4 (Antibody Buffer)
A5 (Capture Antibody, 100 µg/ml)*
A6 (Detection Antibody, 200 µg/ml)*
A7 (Developing Solution)
A8 (Stop Solution)
30% H2O2 Solution
Positive Control (Etoposide, 10 mM)*
Microplates
User Guide
* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.
