We recommend using a newer version of this product: Epigenase HDAC Activity/Inhibition Direct Assay Kit (Fluorometric)

The EpiQuik™ HDAC Activity/Inhibition Assay Kit (Fluorometric) is a ready-to-use set of essential components needed to measure HDAC activity/inhibition safely and quickly. It is suitable for measuring HDAC activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.

WHY CHOOSE THE EPIQUIK™ HDAC ACTIVITY/INHIBITION ASSAY KIT (FLUOROMETRIC)?

• Fast procedure, which can be finished within 3 hours.
• Innovative fluorometric assay without the use of radioactivity, extraction, and chromatography.
• Direct measurement of HDAC activity and inhibition with no use of lysyl endopeptidase, thereby avoiding the false inhibitory effect on HDACs and allowing more accurate measurement.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ HDAC Activity/Inhibition Assay Kit (Fluorometric) is designed for measuring total HDAC activity/inhibition. In an assay with this kit, the unique acetylated histone substrate is stably captured on the strip wells. Active HDACs bind to and deacetylate histone substrate. The remaining un-deacetylated substrate can be recognized with a high affinity acetylated histone antibody. The ratio or amount of the un-deacetylated histone, which is inversely proportional to HDAC enzyme activity, can then be fluorometrically quantified.

SCHEMATIC PROCEDURE:

H1 (10X Wash Buffer)
H2 (HDAC Assay Buffer)
H3 (Biotinylated HDAC Substrate)*
H4 (HDAC Inhibitor, 0.5 mM)*
H5 (HDAC Standard, 20 µg/ml)*
H6 (Capture Antibody, 1000 µg/ml)*
H7 (Detection Antibody, 200 µg/ml)*
Fluoro Developer
8-Well Assay Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.