The EpiQuik™ HDAC Activity/Inhibition Assay Kit (Fluorometric) is designed for measuring total HDAC activity/inhibition. In an assay with this kit, the unique acetylated histone substrate is stably captured on the strip wells. Active HDACs bind to and deacetylate histone substrate. The remaining un-deacetylated substrate can be recognized with a high affinity acetylated histone antibody. The ratio or amount of the un-deacetylated histone, which is inversely proportional to HDAC enzyme activity, can then be fluorometrically quantified.
H1 (10X Wash Buffer)
H2 (HDAC Assay Buffer)
H3 (Biotinylated HDAC Substrate)*
H4 (HDAC Inhibitor, 0.5 mM)*
H5 (HDAC Standard, 20 µg/ml)*
H6 (Capture Antibody, 1000 µg/ml)*
H7 (Detection Antibody, 200 µg/ml)*
8-Well Assay Strip (with Frame)
* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.