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PRDM14 Polyclonal Antibody

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Application: ChIP, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-2014-05050 µg $229.00 
A-2014-100100 µg $399.00 
Availability: Usually Ships in 3-4 Days 
Product Overview

Background 
This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody to PRDM14

Purification
Affinity purified

Immunogen 
Recombinant protein of human PRDM14

Specificity
Human, mouse and rat

Isotype
IgG

Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Storage
Store at -20ºC (regular) or -80ºC (long term). Avoid freeze / thaw cycles.

Alternative Names
MGC59730 antibody, PFM 11 antibody, PR domain zinc finger protein 14 antibody, PR domain-containing protein 14 antibody, PRD14_HUMAN antibody, PFM11

Application
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:20 - 1:100, CHIP: 1:20 - 1:100

Fig. 1. Western blot analysis of extracts of various cell lines, using PRDM14 antibody.

Fig. 2. Western blot analysis of various groups from PA-1 cell line, using PRDM14 antibody. ShRNA-1, shRNA-2, shRNA-3, shRNA-4, shRNA-5, shRNA-6 show knock-down samples which are transfected by different shRNAs targeting PRDM14. Control represents the untreated one.

 Fig. 3. Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using PRDM14 antibody and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

User Guide & MSDS

[User Guide]*
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