This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.
Rabbit polyclonal antibody to PRDM14
Recombinant protein of human PRDM14
Human, mouse and rat
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Store at -20ºC (regular) or -80ºC (long term). Avoid freeze / thaw cycles.
MGC59730 antibody, PFM 11 antibody, PR domain zinc finger protein 14 antibody, PR domain-containing protein 14 antibody, PRD14_HUMAN antibody, PFM11
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:20 - 1:100, CHIP: 1:20 - 1:100
Fig. 1. Western blot analysis of extracts of various cell lines, using PRDM14 antibody.
Fig. 2. Western blot analysis of various groups from PA-1 cell line, using PRDM14 antibody. ShRNA-1, shRNA-2, shRNA-3, shRNA-4, shRNA-5, shRNA-6 show knock-down samples which are transfected by different shRNAs targeting PRDM14. Control represents the untreated one.
Fig. 3. Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using PRDM14 antibody and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.