The EpiQuik™ Histone Demethylase (H3-K9 Specific) Activity/Inhibition Fast Assay Kit is a convenient set of tools that allows the experimenter to measure activity/inhibition of H3-K9 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase (H3-K9 specific) activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.

WHY CHOOSE THE EPIQUIK™ HISTONE DEMETHYLASE (H3-K9 SPECIFIC) ACTIVITY/INHIBITION FAST ASSAY KIT?

• Fast one-step assay, which can be finished within 1.5 hours.
• Innovative fluorescent assay without the need for radioactivity, extraction, and chromatography.
• Measurement of HDM (H3-K9 specific) activity and inhibition through quantifying by-product of the enzyme reaction, which make the assay highly sensitive.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Histone Demethylase (H3-K9 Specific) Activity/Inhibition Fast Assay Kit is designed for measuring total histone demethylase (H3-K9 specific) activity/inhibition. In the assay with this kit, the unique methylated histone H3-K9 substrate is incubated with nuclear extracts or purified enzymes. Formaldehyde generated from the enzyme-substrate reaction reacts with a detection reagent to form a fluorescent substance. The ratio or amount of the formaldehyde, which is proportional to HDM enzyme activity, can then be fluorometrically quantified.

SCHEMATIC PROCEDURE:

HL1 (HDM Assay Buffer)
HL2 (HDM Substrate)*
HL3 (HDM Standard, 1 mM)*
HL4 (Fluoro-Developer)
HL5 (Fluoro-Enhancer)
8-Well Assay Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.