Sitewide 2017 Summer Sale Site-wide summer sale! Take 10% off your entire order when using code E1707SUN. » Learn More
Epigentek Home
 
Epigentek Free Shipping

EpiQuik Histone Demethylase (H3K9 Specific) Activity/Inhibition Fast Assay Kit

Share:

Citations (1) | Write a Review
Schematic procedure for using the EpiQuik Histone Demethylase (H3K9 Specific) Activity/Inhibition Fast Assay Kit.
HDM (H3-K9 specific) activity measured from HeLa cells using the EpiQuik™ Histone Demethylase (H3-K9 Specific) Activity/Inhibition Fast Assay Kit.
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: Histone Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3077-4848 assays $277.00 
P-3077-9696 assays $493.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Histone Demethylase (H3-K9 Specific) Activity/Inhibition Fast Assay Kit is a convenient set of tools that allows the experimenter to measure activity/inhibition of H3-K9 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase (H3-K9 specific) activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria. The kit has the following advantages:

  • Fast one-step assay, which can be finished within 1.5 hours.
  • Innovative fluorescent assay without the need for radioactivity, extraction, and chromatography.
  • Measurement of HDM (H3-K9 specific) activity and inhibition through quantifying by-product of the enzyme reaction, which make the assay highly sensitive.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Histone Demethylase (H3-K9 Specific) Activity/Inhibition Fast Assay Kit is designed for measuring total histone demethylase (H3-K9 specific) activity/inhibition. In the assay with this kit, the unique methylated histone H3-K9 substrate is incubated with nuclear extracts or purified enzymes. Formaldehyde generated from the enzyme-substrate reaction reacts with a detection reagent to form a fluorescent substance. The ratio or amount of the formaldehyde, which is proportional to HDM enzyme activity, can then be fluorometrically quantified.

 
Fig. 1. Schematic procedure for using the EpiQuik Histone Demethylase (H3K9 Specific) Activity/Inhibition Fast Assay Kit.

Fig. 2. HDM (H3-K9 specific) activity measured from HeLa cells using the EpiQuik™ Histone Demethylase (H3-K9 Specific) Activity/Inhibition Fast Assay Kit.

Product Components

HL1 (HDM Assay Buffer)
HL2 (HDM Substrate)*
HL3 (HDM Standard, 1 mM)*
HL4 (Fluoro-Developer)
HL5 (Fluoro-Enhancer)
8-Well Assay Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Guan M et. al. (June 2017). Fatty acid synthase reprograms the epigenome in uterine leiomyosarcomas. PLoS One. 12(6):e0179692.

Epigentek Product Reviews
Related Products

Researchers Also Purchased
EpiQuik Histone Demethylase (H3K4 Specific) Activity/Inhibition Assay Kit
EpiQuik Histone Demethylase (H3K4 Specific) Activity/Inhibition Assay Kit
EpiQuik Nuclear Extraction Kit
EpiQuik Nuclear Extraction Kit
Epigenase LSD1 Demethylase Activity/Inhibition Assay Kit (Fluorometric)
Epigenase LSD1 Demethylase Activity/Inhibition Assay Kit (Fluorometric)
Epigenase JMJD2 Demethylase Activity/Inhibition Assay Kit (Fluorometric)
Epigenase JMJD2 Demethylase Activity/Inhibition Assay Kit (Fluorometric)
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.