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Histone H3K36me3 (H3K36 Trimethyl) Polyclonal Antibody


Citations (4) | Write a Review
Application: ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-4042-02525 µg $139.00 
A-4042-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.

1 mg/ml

Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the trimethylated histone H3 at K36, ChIP-grade

Affinity purification

Human, mouse, rat and broad range


PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles.

Alternative Names
HIST1H3J; H3/j ;H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I;Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3; H3K36me3 antibody; H3K36m3 antibody

WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:20 - 1:100, ChIP-seq: 1:20 - 1:100 


Fig. 6. Immunohistochemistry of paraffin-embedded human rectal tissue using H3K36me3 Polyclonal Antibody at dilution of 1:200 (x400 lens). 

Fig. 7. Immunohistochemistry of paraffin-embedded mouse brain tissue using H3K36me3 Polyclonal Antibody at dilution of 1:200 (x400 lens). 



Fig. 8. Immunofluorescence analysis of 293T cell using H3K36me3 Polyclonal Antibody. Blue: DAPI for nuclear staining.


Fig. 9. Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using TriMethyl-Histone H3-K36 antibody and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Fig. 1. Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K36me3 Polyclonal Antibody.

Fig. 2. Dot-blot analysis of all sorts of methylation peptides using H3K36me3 Polyclonal Antibody.

Fig. 3. Immunohistochemistry of paraffin-embedded rat lung tissue using H3K36me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 4. Immunohistochemistry of paraffin-embedded rat spleen tissue using H3K36me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 5. Immunohistochemistry of paraffin-embedded human lung cancer tissue using H3K36me3 Polyclonal Antibody at dilution of 1:200 (x400 lens).

User Guide & MSDS

[User Guide]*
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Product Citations

Onyango DO et. al. (April 2016). Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination. Nucleic Acids Res.

Creamer KM et. al. (June 2014). The Mi-2 Homolog Mit1 Actively Positions Nucleosomes within Heterochromatin To Suppress Transcription. Mol Cell Biol. 34(11):2046-61.

Schroeder EA et. al. (June 2013). Epigenetic silencing mediates mitochondria stress-induced longevity. Cell Metab. 17(6):954-64.

Ma P et. al. (February 2012). Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A. 109(8):E481-9.

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