The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.
Rabbit polyclonal antibody to HAT1, ChIP-grade
Mouse, rat, human
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20ºC (regular) or -80ºC (long term). Avoid freeze / thaw cycles.
Histidine aminotransferase 1 antibody, Histone acetyltransferase 1 antibody, Histone acetyltransferase type B catalytic subunit antibody, KAT1 antibody, KAT1
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:20 - 1:100
Fig. 1. Western blot analysis of extracts of various cell lines, using HAT1 antibody.
Fig. 2. Immunofluorescence analysis of U2OS cell using HAT1 antibody.