HAT1 Polyclonal Antibody

Background
The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody to HAT1, ChIP-grade

Purification
Affinity purified

Specificity
Mouse, rat, human

Isotype 
IgG

Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage
Store at -20ºC (regular) or -80ºC (long term). Avoid freeze / thaw cycles.

Alternative Names
Histidine aminotransferase 1 antibody, Histone acetyltransferase 1 antibody, Histone acetyltransferase type B catalytic subunit antibody, KAT1 antibody, KAT1

Application 
WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:20 - 1:100

Fig. 1. Western blot analysis of extracts of various cell lines, using HAT1 antibody.

Fig. 2. Immunofluorescence analysis of U2OS cell using HAT1 antibody.