Amazon Gift Card Offer Get a free Amazon.com® gift card with every EpiGentek order! » Learn More
Epigentek Home
 
Epigentek Free Shipping

Histone H4K20me2 (H4K20 Dimethyl) Polyclonal Antibody

Share:

Write a Review
Application: ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-4047-02525 µg $139.00 
A-4047-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background 
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody to the dimethylated histone H4 at K20, ChIP-grade

Purification
Affinity purified

Immunogen
A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4

Specificity
Human, mouse, rat and broad range

Isotype
IgG

Formulation 
PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Storage
Store at -20ºC (regular) or -80ºC (long term). Avoid freeze / thaw cycles

Alternative Names
H4K20me2 antibody: H4K20m2 antibody:H4; H4/n; H4F2; H4FN; FO108; HIST2H4; H4K20me1 antibody; H4K20m1 antibody

Application  
WB: 1:500 - 1:2000, IF: 1:50 - 1:200, IP: 1:50 - 1:200, CHIP: 1:50 - 1:200

Fig. 1. Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using Histone H4K20 Dimethyl Polyclonal Antibody.

Fig. 2. Dot-blot analysis of all sorts of methylation peptides using Histone H4K20 Dimethyl Polyclonal Antibody.

Fig. 3. Immunohistochemistry of paraffin-embedded rat testis tissue using Histone H4K20 Dimethyl Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 4. Immunohistochemistry of paraffin-embedded rat brain tissue using Histone H4K20 Dimethyl Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 5. Immunohistochemistry of paraffin-embedded human thyroid cancer tissue using Histone H4K20 Dimethyl Polyclonal Antibody at dilution of 1:200 (x400 lens).

Fig. 6. Immunofluorescence analysis of 293T cell using Histone H4K20 Dimethyl Polyclonal Antibody. Blue: DAPI for nuclear staining.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2016 Epigentek Group Inc. All rights reserved.