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Histone H3K14ac (Acetyl H3K14) Polyclonal Antibody

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Citations (9) | Write a Review
Western blot analysis of extracts of various cell lines, using Histone H3K14ac (Acetyl H3K14) Polyclonal Antibody.
Application: ChIP, ELISA, IF, IHC, IP, WB
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4023-02525 µg $143.00 
A-4023-05050 µg $277.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of histone H3 acetylated on K14, ChIP-grade

Purification
Affinity purification

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Immunogen
A synthetic acetylated peptide corresponding to residues surrounding K14 of human H3

Formulation 
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Storage
Store at -20°C or -80°C. Avoid freeze / thaw cycles.

Alternative Names
H3K14ac antibody; H3K14a antibody;H3t; H3.4; H3/g; H3FT

Application 
WB 1:1000 - 1:3000
IHC 1:200- 1:500
IF 1:500- 1:1000
IP 1:200- 1:500
ChIP 1:50- 1:100

Usage
Research use only

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Srivas S et. al. (September 2017). Epigenetic regulation of neuronal immediate early genes is associated with decline in their expression and memory consolidation in scopolamine-induced amnesic mice. Mol Neurobiol. 54(7):5107-5119.

Kumar D et. al. (May 2017). Effect of perinatal exposure to Bisphenol-A on DNA methylation and histone acetylation in cerebral cortex and hippocampus of postnatal male mice. J Toxicol Sci. 42(3):281-289.

Singh P et. al. (March 2017). Histone Deacetylase 2 Inhibition Attenuates Downregulation of Hippocampal Plasticity Gene Expression during Aging. Mol Neurobiol.

Sugeno N et. al. (November 2016). α-Synuclein enhances histone H3 lysine-9 dimethylation and H3K9me2-dependent transcriptional responses. Sci Rep. 6:36328.

Han L et. al. (October 2015). Sirt6 depletion causes spindle defects and chromosome misalignment during meiosis of mouse oocyte. Sci Rep. 5:15366.

Singh P et. al. (May 2015). Hippocampal chromatin modifying enzymes are pivotal for scopolamine- induced synaptic plasticity gene expression changes and memory impairment. J Neurochem.

Zhang L et. al. (March 2014). Sirt2 functions in spindle organization and chromosome alignment in mouse oocyte meiosis. FASEB J. 28(3):1435-45.

Zou C et. al. (March 2013). SCF(Fbxw15) mediates histone acetyltransferase binding to origin recognition complex (HBO1) ubiquitin-proteasomal degradation to regulate cell proliferation. J Biol Chem. 288(9):6306-16.

Ma P et. al. (February 2012). Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A. 109(8):E481-9.

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