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PRMT3 Polyclonal Antibody

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Western blot
All lanes: PRMT3 Polyclonal Antibody at 7 ug/ml + Mouse brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 60, 53 kDa
Observed band size: 60 kDa

Immunohistochemistry of paraffin-embedded human brain tissue using PRMT3 Polyclonal Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human glioma using PRMT3 Polyclonal Antibodyat dilution of 1:100
Immunofluorescent analysis of MCF-7 cells using PRMT3 Polyclonal Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Application: ELISA, IF, IHC, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human, Mouse
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-3003-02020 µg $59.00 
A-3003-05050 µg $199.00 
A-3003-100100 µg $391.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
PRMTs (Protein Arginine N-Methyltransferases) can monomethylate or dimethylate arginine residues by transferring methyl groups from S-adenoxylmethionine to the guanidine nitrogen of arginine. PRMT3, known as Protein Arginine N-Methyltransferase 3, is involved in the maturation of the ribosome 80S and acts on 40S (eukaryotic small ribosomal subunit) rpS2 (ribosomal protein S2). Overall, PRMT3 acts at the cellular level of the subunits of ribosomes, which read RNA.

Description
PRMT3 Polyclonal Antibody. Unconjugated. Raised in: Rabbit

Formulation
Liquid.
0.03% Proclin 300. 50% Glycerol, 0.01M PBS, PH 7.4

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
O60678

Purification
Protein G purified

Immunogen
Recombinant human Protein arginine N-methyltransferase 3 protein (311-529AA)

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Protein arginine N-methyltransferase 3 (EC 2.1.1.-) (Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3), PRMT3, HRMT1L3

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

 

VCU0006

User Guide & MSDS

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