Concentration
1 mg/ml

Description
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the trimethylated histone H4 at K20, ChIP-grade

Specificity
Detects histone H4 only when trimethylated at K20 in mouse, rat, and human. Broad species to cross-reactivity is also expected.

Isotype
IgG

Formulation
PBS (pH 7.5), 150 mM NaCl, 30% glycerol

Storage
-20°C, stable for 1 year from the date of shipment. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Application
WB: 1:1000-1:2000, IF: 1:100-1:500, IH: 1:100-1:500, ELISA: 1:1000-1:2000, IP: 2 μg/10⁶ cells

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Diehl, F. et. al. (March 2010). Cardiac deletion of Smyd2 is dispensable for mouse heart development. PLoS One. 5(3): e9748. PubMed Abstract