The EpiQuik™ DNMT3B Activity/Inhibitor Screening Assay Core Kit is a convenient set of tools that allows the experimenter to selectively screen DNMT3B activity/inhibitors in an ELISA-like format. The selective inhibition of DNMT3B may lead to demethylation and expression of silenced tumor suppressor genes. Thus, the selective DNMT3B inhibitors could be a new addition to cancer therapeutic agents. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3B activity/inhibitor screening experiment without the need for radioactivity or any special equipment.

WHY CHOOSE THE EPIQUIK™ DNMT3B ACTIVITY/INHIBITOR SCREENING ASSAY CORE KIT?

• Very rapid procedure, which can be completed within 3 hours.
• Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Extremely simple, reliable, and consistent assay conditions.

The EpiQuik™ DNMT3B Activity/Inhibitor Screening Assay Core Kit is designed for screening Dnmt 3B inhibitors. In an assay with this kit, the unique cytosine-rich DNA substrate is stably coated on the strip wells. These wells are specifically treated to have a high DNA absorption ability. A DNMT3B (not included) enzyme transfers a methyl group to cytosine from Adomet to methylate DNA substrate. The methylated DNA can be recognized with an anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.

SCHEMATIC PROCEDURE:

MT1 (10X Wash Buffer)
MT2 (Dnmt Assay Buffer)
MT3 (Adomet)*
MT5 (Capture Antibody)*
MT6 (Detection Antibody)*
MT7 (Developing Solution)
MT8 (Stop Solution)
8-Well Substrate-Coated Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.