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EpiQuik DNMT1 Activity/Inhibitor Screening Assay Core Kit

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For screening DNMT1 inhibitors in an ELISA-like format

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Schematic procedure for using the EpiQuik DNMT1 Activity/Inhibitor Screening Assay Core Kit.
DNMT1 inhibition: Recombinant DNMT1 was incubated with substrate and inhibitors. DNMT1 activity was measured in the presence or absence of inhibitors.
Input Type: Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3006A-4848 assays $256.00 
P-3006A-9696 assays $483.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ DNMT1 Activity/Inhibitor Screening Assay Core Kit is a convenient set of tools that allows the experimenter to selectively screen DNA methyltransferase 1 activity/inhibitors in an ELISA-like format. The selective inhibition of DNMT1 may lead to demethylation and expression of silenced tumor suppressor genes. Thus, the selective DNMT1 inhibitors could be a new addition to cancer therapeutic agents. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 activity/inhibitor screening experiment without the need for radioactivity or any special equipment. The kit has the following advantages:

  • Very rapid procedure, which can be completed within 3 hours.
  • Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Extremely simple, reliable, and consistent assay conditions.

Note: This "core" kit does not come with enzymes included.

Principle & Procedure
The EpiQuik™ DNMT1 Activity/Inhibitor Screening Assay Kit is designed for screening Dnmt 1 inhibitors. In an assay with this kit, the unique cytosine-rich DNA substrate is stably coated on the strip wells. These wells are specifically treated to have a high DNA absorption ability. A DNMT1 enzyme (not included) transfers a methyl group to cytosine from Adomet to methylate the DNA substrate. The methylated DNA can be recognized with an anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.

 
Fig. 1. Schematic procedure for using the EpiQuik DNMT1 Activity/Inhibitor Screening Assay Core Kit.

Fig. 2. DNMT1 inhibition: Recombinant DNMT1 was incubated with substrate and inhibitors. DNMT1 activity was measured in the presence or absence of inhibitors.

Product Components

MO1 (10X Wash Buffer)
MO2 (Dnmt Assay Buffer)
MO3 (Adomet)*
MO5 (Capture Antibody)*
MO6 (Detection Antibody)*
MO7 (Developing Solution)
MO8 (Stop Solution)
8-Well Substrate-Coated Strip (with Frame)
User guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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