The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is a convenient package of tools that allows the experimenter to measure Dnmt activity or inhibition at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt activity/inhibition experiment without the need for radioactivity or any special equipment. The EpiQuik™ DNA Methyltransferase Activity/Inhibition (Dnmt) Assay Kit is suitable for measuring Dnmt activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.

WHY CHOOSE THE EPIQUIK™ DNA METHYLTRANSFERASE ACTIVITY/INHIBITION ASSAY KIT?

• Very rapid procedure, which can be completed within 3 hours.
• Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Extremely simple, reliable, and consistent assay conditions.

The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is designed for measuring total Dnmt activity (de novo, maintenance). In an assay with this kit, the unique cytosine-rich DNA substrate is stably coated on the strip wells. These wells are specifically treated to have a high DNA absorption ability. Dnmt enzymes transfer methyl group to cytosine from Adomet to methylate DNA substrate. The methylated DNA can be recognized with anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.

SCHEMATIC PROCEDURE:
	Analysis of Dnmt activity in Hela nuclear extract.

M1 (10x wash buffer)
M2 (Dnmt assay buffer)
M3 (Adomet, 8 mM)*
M4 (Dnmt positive control)*
M5 (capture antibody)*
M6 (detection antibody 200 µg/ml)*
M7 (developing solution)
M8 (stop solution)
8 well substrate-coated strip (with frame)
User guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

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