DNA Bisulfite Modification & Methylation   Methylamp DNA Modification Kit Methylamp Coupled DNA Isolation & Modification Kit Methylamp 96 DNA Modification Kit Methylamp One-Step DNA Modification Kit Methylamp Whole Cell Bisulfite Modification Kit Methylamp Universal Methylated DNA Kit Methylamp Universal Methylated DNA Preparation Kit Catalog Number P-1001 P-1002 P-1008 P-1010 P-1016 P-1011 P-1019 Properties Input Material DNA Cells, tissues, blood DNA DNA Cells, tissues, blood Methylated DNA DNA Lowest amount of Input 50 pg 500 cells/≤ 1 ul blood 100 pg 50 pg ≤ 100 cells/≤ 1 µl blood 100 pg 1 ng Bisulfite Processing Time 1.5 h 1.5 h 1.5 h 1.5 h 1.5 h 1.5 h   Total Processing Time ≤ 2 h ≤ 2.5 h ≤ 2.5 h < 2 h < 3 h < 2 h 4-16 h Elution Volume 8-20 µl 8-20 µl 20-40 µl 8-20 µl 8-20 µl 8-20 µl 10-100 µl Conversion Efficiency 99.9% 99.9% 99.5% 99.5% 99.9%     Desulphonation/clean-up ✓ ✓ ✓ ✓ ✓     Bisulfite DNA recovery >75% >75% >75% >75% >85% 75% 85% Format Single vial ✓ ✓   ✓ ✓ ✓ ✓ 96-well plate     ✓         Rapid spin column ✓ ✓ ✓ ✓ ✓ ✓

Methylated DNA Quantification   Methylamp Global DNA Methylation Quantification Kit Methylamp Global DNA Methylation Quantification Ultra Kit SuperSense Methylated DNA Quantification Kit Catalog Number P-1014 P-1014B P-1021 Properties Input Material ssDNA/dsDNA ssDNA/dsDNA ssDNA/dsDNA Maximum Amount of Input 400 ng 200 ng 200 ng Minimum Amount of Input 100 ng 20 ng 5 ng Processing Time 4 h 3 h 2.5 h Detection Limit (Methylated DNA) 1 ng 0.2 ng 0.05 ng Specificity (meDNA/unDNA Detection Ratio) > 60:1 > 40:1 > 40:1 Format Colorimetric ✓ ✓   Fluorometric     ✓ Strip-well Microplate ✓ ✓ ✓ Application Purpose Methylated DNA quantification. Excellent for relative comparison between samples. Good for measurement of absolute amount in an individual sample. Methylated DNA quantification. Excellent for relative comparison between samples. Good for measurement of absolute amount in an individual sample. Methylated DNA quantification. Excellent for relative comparison between samples. Good for measurement of absolute amount in an individual sample.

DNA Methyltransferase   EpiQuik Dnmt Activity/ Inhibition Assay Kit EpiQuik Dnmt Activity/ Inhibition Assay Kit (Fluorometric) EpiQuik Dnmt1 Activity/ Inhibitor Screening Assay Kit EpiQuik Dnmt3B Activity/ Inhibitor Screening Assay Kit EpiQuik Dnmt1 Assay Kit EpiQuik Dnmt3A Assay Kit EpiQuik Dnmt3B Assay Kit Catalog Number P-3001 P-3004 P-3006 P-3007 P-3011 P-3012 P-3013 Properties Input Material Nuclear Extract/Purified Enzyme Nuclear Extract/Purified Enzyme Purified Enzyme Purified Enzyme Nuclear Extract/Purified Enzyme Nuclear Extract/Purified Enzyme Nuclear Extract/Purified Enzyme Amount of Input 5-20 µg/20-200 ng 5-20 µg/5-200 ng 50-100 ng 50-100 ng 2-20 µg/2-100 ng 2-20 µg/2-100 ng 2-20 µg/2-100 ng Positive Control ✓ ✓     ✓ ✓ ✓ Purified Enzyme for Assay     ✓ ✓       Processing Time 3-4 h 3-4 h 3-4 h 3-4 h 3 h 3 h 3 h Detection Limit (Purified Dnmt1/3b) 10 ng 2 ng     2 ng 1 ng 2 ng Format Colorimetric ✓   ✓ ✓ ✓ ✓ ✓ Fluorometric   ✓           Strip-well Microplate ✓ ✓ ✓ ✓ ✓ ✓ ✓ Application Purpose Total Dnmt activity measurement. Also suitable for Dnmt inhibition measurement after in vivo/in vitro treatment with Dnmt inhibitors. Total Dnmt activity measurement. Also suitable for Dnmt inhibition measurement after in vivo/in vitro treatment with Dnmt inhibitors. Dnmt1 inhibitor screening. Dnmt 3b inhibitor screening. Dnmt1 protein level quantification. Dnmt 3a protein level quantification. Dnmt 3b protein level quantification.

DNA Preparation   FitAmp General Tissue Section DNA Isolation Kit FitAmp Plasma/Serum DNA Isolation Kit FitAmp Gel DNA Isolation Kit FitAmp Paraffin Tissue Section DNA Isolation Kit DNA Concentrator Kit FitAmp Urine DNA Isolation Kit FitAmp Blood and Cultured Cell DNA Extraction Kit Catalog Number P-1003 P-1004 P-1007 P-1009 P-1006 P-1017 P-1018 Properties Input Material Tissue/Tissue Section Plasma/Serum Gel DNA Paraffin Tissues DNA Urine Blood/Cultured Cells Maximum DNA Amount/Prep 5 µg ≤ 5 µg ≤ 2 µg ≤ 5 µg ≤ 5 µg ≤ 5 µg ≤ 5 µg ≤ 5 µg Total Processing Time ≤ 2 h ≤ 15 min ≤ 15 min < 2 h ≤ 3 min ≤ 30 min ≤ 30 min Elution Volume 8-20 µl 8-20 µl 20-40 µl 8-20 µl 8-20 µl 8-20 µl 8-20 µl % DNA Yield > 70% > 70% > 85% > 70% > 85% > 75% > 75% Format Single Vial ✓ ✓ ✓ ✓ ✓ ✓ ✓ Proteinase K ✓ ✓   ✓   ✓ ✓ Rapid Spin Column ✓ ✓ ✓ ✓ ✓ ✓ ✓ Application Purpose DNA Isolation DNA Isolation Gel DNA Isolation DNA Isolation DNA Concentrating DNA Isolation DNA Isolation Fresh/Frozen Tissue ✓             FFPE Tissue       ✓       Tissue Sections ✓             FFPE Tissue Sections       ✓       Cultured Cells             ✓ Whole Blood             ✓ Buccal Cells/Swabs             ✓ Buffy Coat             ✓ Urine           ✓   Semen           ✓ ✓ Plasma/Serum   ✓

Protein Extraction   EpiQuik Nuclear Extraction Kit I EpiQuik Nuclear Extraction Kit II EpiQuik Whole Cell Extraction Kit EpiQuik Total Histone Extraction Kit Catalog Number OP-0002-1 OP-0002-2 OP-0003-100 OP-0006 Properties Input Material Tissue/Cells Tissue/Cells Cells Tissue/Cells Input Amount Varied Varied Varied Varied Total Processing Time ≤ 1 h ≤ 1 h ≤ 45 min < 1 h Extracted Proteins Nuclear Protein Nucleic Acid-Free Whole Cell Protein Histone Protein Protein Yield 5 µg/10 mg tissue or 1 million cells 5 µg/10 mg tissue or 1 million cells 10 µg/10 mg tissue or 1 million cells 0.4 µg/100 mg tissue or 1 million cells Nucleic Acid with Protein Yes None, or Little Yes Yes Enzyme Activity Yes No Yes No Application Purpose Enzyme Activity Assay, Protein Detection Protein Detection Enzyme Activity Assay, Protein Detection Histone Detection Fresh/Frozen Tissue ✓ ✓ ✓ ✓ Cultured Cells ✓ ✓ ✓ ✓ Whole Blood ✓ ✓ ✓ ✓ Buccal Cells/Swabs ✓ ✓ ✓ ✓ Buffy Coat ✓ ✓ ✓ ✓ Urine ✓ ✓ ✓ ✓ Semen ✓ ✓ ✓ ✓