(For Nucleic Acid-Free Extraction)
The EpiQuik™ Nuclear Extraction Kit provides the simple and selective method for extracting nuclear proteins used for a variety of applications. These applications may include western blotting, protein-DNA binding assays, nuclear enzyme assays, and the others requiring optimized or nucleic acid-free nuclear proteins. The EpiQuik™ Nuclear Extraction Kits are also specifically designed to meet the requirements of nuclear extracts used in various EpiQuik™ assays. The EpiQuik™ Nuclear Extraction Kits can be used to extract nuclear proteins from mammalian cells and tissue samples. The EpiQuik™ Nuclear Extraction Kits include kit I for regular nuclear extraction and kit II for nucleic acid-free nuclear extraction. The EpiQuik™ Nuclear Extraction Kits have the fastest procedure that can be finished within 60 minutes.

 

Note: This item was formerly catalog no. OP-0002-2 (OP00022, OP-00022, OP0002-2)

The EpiQuik™ Nuclear Extraction Kit II simply applies our proprietary nucleic acid-free protein isolation buffers to cell/tissues. After treatment with pre-lysis, lysis and nucleic acid eliminating solutions, the nucleic acid-free nuclear proteins are easily extracted for immediate use or storage at proper conditions.

NP1 (10X Pre-Extraction Buffer)
NP2 (Extraction Buffer)
NP3 (Extraction Pre-Cleaner)
NP4 (Extraction Cleaner)
1000X DTT Solution
1000X Protease Inhibitor Cocktail (PIC)
User Guide

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]