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Base Cat #OP-0022

EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)

5.01 Review|27 Citations

For isolation of nucleic acid-free nuclear proteins from cells or tissues

Input Type: Unisolated Samples
Research Area: DNA Methylation, Histone Acetylation, Histone Methylation, Sumoylation
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Application Guarantee for AntibodiesRecombinant Rabbit Monoclonal Abs
Catalog No.SizePriceQty
OP-0022-100100 extractions $198.00 
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Order within 10 hr 18 min & get it by Tuesday, September 29th  

Product Overview

The EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free) is a complete set of optimized reagents to provide a simple and selective method for isolating nuclear proteins free of nucleic acids attached, to be used in a variety of applications in just 60 minutes. These applications may include Western blot, protein-DNA binding assays, nuclear enzyme assays, and any other procedures requiring optimized nucleic acid-free nuclear proteins but not enzymatic activity. The protocol is fast and easy to use and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples. Some of the kit's highlighted features include the following:

  • Pre-optimized and simple step-wise 1-hour protocol.
  • Conveniently includes all essential reagents to carry out a nucleic acid-free nuclear extraction.
  • Standardized procedure for reproducible results.

Principle & Procedure
The EpiQuikâ„¢ Nuclear Extraction Kit II (Nucleic Acid-Free) simply applies our proprietary nuclear protein isolation buffers to cells/tissues. After pre-lysis and lysis buffers are treated, the nucleic acid-free nuclear proteins are easily extracted for immediate use or storage under proper conditions.

Note: This item was formerly catalog no. OP-0002-2 (OP00022, OP-00022, OP0002-2)

Datasheet & SDS

PDF
Datasheet/User Guide: OP-0022 If the above file is not available online, you can request a copy by contacting us.
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Product FAQ  

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Product Citations

  1. Wang X et. al. (September 2026). Mechanical pressure on endothelial cells mediates remote ischaemic preconditioning-induced neuroprotection via miR-126. Exp Physiol. PubMed: 42702849
  2. Elsayed MA et. al. (March 2025). Protective Effects of Galangin Against Cyclophosphamide-Induced Cardiotoxicity via Suppressing NF-κB and Improving Mitochondrial Biogenesis. J Biochem Mol Toxicol. 39(3):e70193.
  3. Nakazawa Y et. al. (March 2024). Delivery of a BET protein degrader via a CEACAM6-targeted antibody-drug conjugate inhibits tumour growth in pancreatic cancer models. Nat Commun. 15(1):2192.
  4. Pirola CJ et. al. (February 2024). Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease. EBioMedicine. 101:104996.
  5. Possamai-Della T et. al. (May 2023). Prenatal Stress Induces Long-Term Behavioral Sex-Dependent Changes in Rats Offspring: the Role of the HPA Axis and Epigenetics. Mol Neurobiol.
View all publications (27)

Customer Reviews

Write a Review
Rating ★★★★★5/5 by p*******@stanford.edu Verified Purchase
Reviewed on: Monday 19 August, 2024
Application Description
I used this kit to isolate histones for western blot experiments. I used H3K27Ac and total Histone H3 antibody.
  1. Wang X et. al. (September 2026). Mechanical pressure on endothelial cells mediates remote ischaemic preconditioning-induced neuroprotection via miR-126. Exp Physiol. PubMed: 42702849
  2. Elsayed MA et. al. (March 2025). Protective Effects of Galangin Against Cyclophosphamide-Induced Cardiotoxicity via Suppressing NF-κB and Improving Mitochondrial Biogenesis. J Biochem Mol Toxicol. 39(3):e70193.
  3. Nakazawa Y et. al. (March 2024). Delivery of a BET protein degrader via a CEACAM6-targeted antibody-drug conjugate inhibits tumour growth in pancreatic cancer models. Nat Commun. 15(1):2192.
  4. Pirola CJ et. al. (February 2024). Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease. EBioMedicine. 101:104996.
  5. Possamai-Della T et. al. (May 2023). Prenatal Stress Induces Long-Term Behavioral Sex-Dependent Changes in Rats Offspring: the Role of the HPA Axis and Epigenetics. Mol Neurobiol.
  6. Arora L et. al. (June 2022). Hypoxia-induced miR-210-3p expression in lung adenocarcinoma potentiates tumor development by regulating CCL2-mediated monocyte infiltration. Mol Oncol.
  7. Barks AK et. al. (January 2022). Developmental iron deficiency dysregulates TET activity and DNA hydroxymethylation in the rat hippocampus and cerebellum. Dev Neurosci.
  8. Liu et. al. (December 2021). Prenatal Iron Deficiency and Choline Supplementation Interact to Epigenetically Regulate Jarid1b and Bdnf in the Rat Hippocampus into Adulthood Nutrients. 13(12):4527.
  9. Yamada K et. al. (January 2021). E7386, a selective inhibitor of the interaction between β-catenin and CBP, exerts antitumor activity in tumor models with activated canonical Wnt signaling. Cancer Res.
  10. Kołodziej-Wojnar P et. al. (December 2020). Alterations in the Genomic Distribution of 5hmC in In Vivo Aged Human Skin Fibroblasts. Int J Mol Sci. 22(1)
  11. He F et. al. (April 2020). LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells. Stem Cell Res Ther. 11(1):163.
  12. Araki M et. al. (April 2020). The role of glucocorticoid receptors in the induction and prevention of hippocampal abnormalities in an animal model of posttraumatic stress disorder. Psychopharmacology (Berl).
  13. Nguyen TD et. al. (December 2018). Female sex hormones activate HERV-K through the OCT4 transcription factor in T47D breast cancer cells. AIDS Res Hum Retroviruses.
  14. Liu et. al. (July 2018). Integrated regulation of class II human endogenous retroviruses in a breast cancer cell line Eighth International Conference on Creationism. 8(16):191-199.
  15. Liu Y et. al. (July 2018). Integrated regulation of class II human endogenous retroviruses in a breast cancer cell line The Proceedings of the International Conference on Creationism. 8(16):191–199.
  16. Yuyan Tan et. al. (April 2018). Upregulation of HDAC2 in Laser Capture Nigral Microglia in Parkinson’s Disease. Neurobiology of Aging.
  17. Yana H et. al. (December 2015). The effects of LSD1 inhibition on self-renewal and differentiation of human induced pluripotent stem cells Exp Cell Res.
  18. Haupt S et. al. (November 2015). Alternative promoter methylation regulates together with Foxp3 transcription of GARP. Biochim Biophys Acta.
  19. Ivankovic D et. al. (October 2015). Mitochondrial and lysosomal biogenesis are activated following PINK1/parkin-mediated mitophagy. J Neurochem.
  20. Wessam F. El-Hadidy et. al. (April 2014). Possible protective effect of procainamide as an epigenetic modifying agent in experimentally induced type 2 diabetes mellitus in rats. Alexandria Journal of Medicine. S2090-5068(14):00028-1. Abstract
  21. Siuda D et. al. (April 2014). Social isolation-induced epigenetic changes in midbrain of adult mice. J Physiol Pharmacol. 65(2):247-55.
  22. Yamashita K et. al. (March 2014). The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation. FEBS Open Bio. 4:347-52.
  23. Wei W et. al. (May 2013). Codelivery of mTERT siRNA and paclitaxel by chitosan-based nanoparticles promoted synergistic tumor suppression. Biomaterials. 34(15):3912-23.
  24. Galoian K et. al. (June 2012). mTORC1 inhibition and ECM-cell adhesion-independent drug resistance via PI3K-AKT and PI3K-RAS-MAPK feedback loops. Tumour Biol. 33(3):885-90.
  25. Harkless R et. al. Microvesicle-Mediated Transfer of DNA Methyltransferase Proteins Results in Recipient Cell Immunosuppression. J Surg Res. 283:368-376. PubMed: 36427447
  26. Hennion V et. al. Shared and distinct effects of mood stabilizers on epigenetic machinery gene expression: an exploratory study in HeLa cells. Eur Neuropsychopharmacol. 110:112836. PubMed: 41955765
  27. Hanafy SM et. al. Irisin-Driven AMPK-PGC-1α Activation Underlies the Renoprotective Effects of Swimming Exercise in Obesity-Induced Kidney Injury. Biomolecules. 16(5) PubMed: 42194075
Rating ★★★★★5/5 by p*******@stanford.edu Verified Purchase
Reviewed on: Monday 19 August, 2024
Application Description
I used this kit to isolate histones for western blot experiments. I used H3K27Ac and total Histone H3 antibody.
Pros Details
Easy to use, requires very less reconstitution steps of components.
Cons Details
None.
Other Thoughts
The technical teams was very responsive and answered my questions promptly.
The data shows H3K27Ac blot showing differential acetylation. The samples were prepared using the EpiQuik Nuclear Extraction Kit
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