The EpiQuik™ Dnmt3A Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of Dnmt3A at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt3A assay experiment. The EpiQuik™ Dnmt3A Assay Kit is suitable for measuring Dnmt amounts quantitatively from fresh tissue and cultured cells of human and mouse.

WHY CHOOSE THE EPIQUIK™ DNMT3A ASSAY KIT?

• The fastest procedure, which can be finished within 3 hours.
• Innovative colorimetric assay, which quantitatively measure Dnmt3A amount without the need for electrophoresis.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Dnmt3A Assay Kit is designed for measuring total Dnmt3A amount from tissues or cells. In an assay with this kit, the unique Dnmt affinity substrate is stably coated on the strip well. The sample is added into the well and Dnmt3A contained in the sample binds to the substrate. The bound Dnmt3A can be recognized with a specific Dnmt3A antibody and colorimetrically quantified through an ELISA-like reaction. The amount of Dnmt3A is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:
	Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total amount of Dnmt was measured.

DA1 (10X Wash Buffer)
DA2 (Assay Buffer)
DA3 (Dnmt3A Standard, 20 µg/ml)*
DA4 (Affinity Antibody, 100 µg/ml)*
DA5 (Detection Antibody, 200 µg/ml)*
DA6 (Developing Solution)
DA7 (Stop Solution)
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

Majid, S. et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-670. PubMed Abstract

Roll, J. D. et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Molecular Cancer. 7: 15. Full PDF Article