The EpiQuik™ Nuclear Extraction Kit provides a simple and selective method along with all necessary reagents for extracting nuclear proteins used for a variety of applications in just 60 minutes. These applications may include western blotting, protein-DNA binding assays, nuclear enzyme assays, and any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples.

The EpiQuik™ Nuclear Extraction Kit is also specifically designed to meet the requirements of nuclear extracts used in various EpiQuik™ assays.

The EpiQuik™ Nuclear Extraction Kit simply applies our proprietary nuclear protein isolation buffers to cell/tissues. After treatment with pre-lysis and lysis buffers, the nuclear proteins are easily extracted for immediate use or storage at proper conditions. 

 

NE1 (10X Pre-Extraction Buffer)
NE2 (Extraction Buffer)
PIC (1000X Protease Inhibitor Cocktail)
1000X DTT Solution
User Guide

1. Should DTT be added into NE1 for tissue extraction?
It is not necessary.

2. How many rpm should be used for centrifuging the cell extract if using desk-top centrifuge?
12,000-14,000 rpm.

3. What should I do if addition of 5 µl NE2 into 1 M of cells can not suspend the nuclei?
Add more NE2, Ex: 10 µl to 1 M nuclei.

4. Can more solution be added for assay reaction if more nuclear extracts will be added?
Yes.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

Li, Y. et. al. (April 2013). Acute myocardial infarction induced functional cardiomyocytes to re-enter the cell cycle. Am J Transl Res. 5(3):327-35. PubMed Abstract

Kauntz, H. et. al. (April 2013). Epigenetic effects of the natural flavonolignan silibinin on colon adenocarcinoma cells and their derived metastatic cells. Oncol Lett. 5(4) :1273-1277. PubMed Abstract

Ding, Z. et. al. (March 2013). Propofol Administration Modulates AQP-4 Expression and Brain Edema After Traumatic Brain Injury. Cell Biochem Biophys. Epub ahead of print. PubMed Abstract

Kano, Y. et. al. (February 2013). Suppression of LFA-1 Expression by Spermine Is Associated with Enhanced Methylation of ITGAL, the LFA-1 Promoter Area. PLoS One. 8(2):e56056. PubMed Abstract

Sanders, Y. et. al. ( 2013). Histone Modifications in Senescence-Associated Resistance to Apoptosis by Oxidative Stress. Redox Biology. 1(1):8-16. Full Article

Zeng, H. et. al. (February 2013). Admixture Mapping in Lupus Identifies Multiple Functional Variants within IFIH1 Associated with Apoptosis, Inflammation, and Autoantibody Production. PLoS Genetics. Full Article

Kim, G. et. al. (February 2013). A Novel Role for IL-27 in Mediating the Survival of Activated Mouse CD4 T Lymphocytes. J Immunol. 190(4) :1510-8. PubMed Abstract

Yamada, D. et. al. (January 2013). Role of crosstalk between interleukin-6 and transforming growth factor-beta 1 in epithelial–mesenchymal transition and chemoresistance in biliary tract cancer. Eur J Cancer. Epub ahead of print. PubMed Abstract

Metes-Kosik, N. et. al. (December 2012). Both selenium deficiency and modest selenium supplementation lead to myocardial fibrosis in mice via effects on redox-methylation balance. Mol Nutr Food Res. 56(12):1812-24. PubMed Abstract

D'Costa, ZJ. et. al. (November 2012). Transcriptional Repression of E-Cadherin by Human Papillomavirus Type 16 E6. PLoS One. 7(11):e48954. PubMed Abstract

Sun, L. et. al. (October 2012). The promoter region of interferon-gamma is hypermethylated in neonatal foals and its demethylation is associated with increased gene expression. Dev Comp Immunol. Epub ahead of print. PubMed Abstract

Yamawaki, Y. et. al. (September 2012). Antidepressant-like effect of sodium butyrate (HDAC inhibitor) and its molecular mechanism of action in the rat hippocampus. World J Biol Psychiatry. 13(6):458-67. PubMed Abstract

Desplats, P. et. al. (September 2012). a-Synuclein Induces Alterations in Adult Neurogenesis in Parkinson Disease Models via p53-mediated Repression of Notch1. J Biol Chem. 287(38):31691-702. PubMed Abstract

Babu, PV. et. al. (September 2012). Epigallocatechin gallate reduces vascular inflammation in db/db mice possibly through an NF-?B-mediated mechanism. Mol Nutr Food Res. 56(9):1424-32. PubMed Abstract

Zopf, S. et. al. (September 2012). Inhibition of DNA methyltransferase activity and expression by treatment with the pan-deacetylase inhibitor panobinostat in hepatocellular carcinoma cell lines. BMC Cancer. PDF

Ramani, K. et. al. (August 2012). Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis. J. Nutr. Epub ahead of print. PubMed Abstract

Samudio-Ruiz, SL. et. al. (March 2012). Increased DNA methyltransferase activity and DNA methylation following Epidermal Growth Factor stimulation in ovarian cancer cells. Epigenetics. 7(3):216-24. PubMed Abstract

Maeda, R. et. al. (2012). Immunostimulatory Activity of Polysaccharides Isolated from Caulerpa lentillifera on Macrophage Cells. Biosci Biotechnol Biochem. 76(3):501-5. PubMed Abstract

Huang, FY. et. al. (March 2012). Helicobacter pylori induces promoter methylation of E-cadherin via interleukin-1ß activation of nitric oxide production in gastric cancer cells. Cancer. Epub ahead of print. PubMed Abstract

Ponomarev, H. et. al. (February 2012). Gene coexpression networks in human brain identify epigenetic modifications in alcohol dependence. J Neurosci. 32(5):1884-97. PubMed Abstract

Neri, F. et. al. (February 2012). Myc Regulates the Transcription of the PRC2 Gene To Control the Expression of Developmental Genes in Embryonic Stem Cells. Mol Cell Biol. 32(4):840-51. PubMed Abstract

Horiuchi A. et. al. (January 2012). Hypoxia upregulates ovarian cancer invasiveness via the binding of HIF-1α to a hypoxia-induced, methylation free hypoxia response element (HRE) of S100A4 gene. Int J Cancer. Epub ahead of print. PubMed Abstract

Galoian, K. et. al. (January 2012). mTORC1 inhibition and ECM-cell adhesion-independent drug resistance via PI3K-AKT and PI3K-RAS-MAPK feedback loops. Tumour Biol. Epub ahead of print. PubMed Abstract

Begum, G. et. al. (January 2012). Epigenetic changes in fetal hypothalamic energy regulating pathways are associated with maternal undernutrition and twinning. FASEB J. Epub ahead of print. PubMed Abstract

Shu, L. et. al. (December 2011). Epigenetic CpG Demethylation of the Promoter and Reactivation of the Expression of Neurog1 by Curcumin in Prostate LNCaP Cells. AAPS J. 13(4):606-14. PubMed Abstract

Trisciuoglio, D. et. al. (November 2011). CPTH6, a thiazole-derivative, induces histone hypoacetylation and apoptosis in human leukemia cells. Clin Cancer Res. Epub ahead of print.  PubMed Abstract

Yang, J. et. al. (November 2011). Stem Cell Gene SALL4 Suppresses Transcription through Recruitment of DNA Methyltransferases. J Biol Chem. Epub ahead of print. PubMed Abstract

Huang, L. et. al. (August 2011). Prevention of transcriptional silencing by a replicator-binding complex consisting of SWI/SNF, MeCP1, and hnRNP C1/C2. Mol Cell Biol. 31(16):3472-84. PubMed Abstract

Cai, H. et. al. (August 2011). Inhibition of melanoma cell proliferation by targeting Wnt/ß-catenin pathway through Sox4 RNA interference. 31(4):656-9. PubMed Abstract

Yamawaki, Y. et. al. (August 2011). Antidepressant-like effect of sodium butyrate (HDAC inhibitor) and its molecular mechanism of action in the rat hippocampus. World J Biol Psychiatry. Epub ahead of print. PubMed Abstract

Geyer, K. et. al. (August 2011). Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni. Nat Commun. 2:424. PubMed Abstract

Fritzenwanger, M. et. al. (2011). Impact of short-term systemic hypoxia on phagocytosis, cytokine production, and transcription factor activation in peripheral blood cells. Mediators Inflamm. 2011:429501. PubMed Abstract

Nandakumar, V. et. al. (April 2011). (-)-Epigallocatechin-3-gallate reactivates silenced tumor suppressor genes, Cip1/p21 and p16INK4a, by reducing DNA methylation and increasing histones acetylation in human skin cancer cells. Carcinogenesis. 32(4): 537-44. PubMed Abstract

Nocchi, L. et. al. (April 2011). Thrombomodulin Is Silenced in Malignant Mesothelioma by a Poly(ADP-ribose) Polymerase-1-mediated Epigenetic Mechanism. J Biol Chem. 286(22): 19478-88. Full PDF Article

Shi, Y. Y. et. al. (April 2011). Diet and Cell Size Both Affect Queen-Worker Differentiation through DNA Methylation in Honey Bees (Apis mellifera, Apidae). PLoS One. 6(4): e18808. PubMed Abstract

Jin, Y. et. al. (March 2011). Homocysteine levels impact directly on epigenetic reprogramming in astrocytes. Neurochem Int. Epub ahead of print. PubMed Abstract

Kawakami, K. et. al. (January 2011). Long interspersed nuclear element-1 hypomethylation is a potential biomarker for the prediction of response to oral fluoropyrimidines in microsatellite stable and CpG island methylator phenotype-negative colorectal cancer. Cancer Science. 102(1): 166-74. PubMed Abstract 

Herrera, F. et. al. (December 2010). Synergistic effect of retinoic acid and cytokines on the regulation of glial fibrillary acidic protein expression. J Biol Chem. 285(50): 38915-22. Full PDF Article

Dusio, G. F. et. al. (November 2010). Stimulation of TLRs by LMW-HA induces self-defense mechanisms in vaginal epithelium. Immunol Cell Biol. Epub ahead of print. PubMed Abstract

Skowronski, K. et. al. (August 2010).Ischemia dysregulates DNA methyltransferases and p16INK4a methylation in human colorectal cancer cells. Epigenetics. 5(6):547-56. PubMed Abstract

Rugg-Gunn, PJ. et. al. (June 2010). Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo. Proc Natl Acad Sci USA. 107(24):10783-90. PubMed Abstract

Chen, F. et. al. (June 2010). Influence of silencing TRAF6 with shRNA on LPS/TLR4 signaling in vitro. Journal of Huazhong University of Science and Technology. 30(3): 278-84. PubMed Abstract

Pandey, M. et. al. (June 2010). Promoter demethylation and chromatin remodeling by green tea polyphenols leads to re-expression of GSTP1 in human prostate cancer cells. International Journal of Cancer. 126(11): 2520-33. PubMed Abstract

Hu, B. et. al. (May 2010). Epigenetic Regulation of Myofibroblast Differentiation by DNA Methylation. Am J Pathol. 177(1): 21-8. PubMed Abstract

Kawada, S. et. al. (May 2010). Increased oxygen tension attenuates acute ultraviolet-B-induced skin angiogenesis and wrinkle formation. Am J Physiol Regul Integr Comp Physiol. 299(2): R694-701. PubMed Abstract

Kastl, L. et. al. (April 2010). Effects of decitabine on the expression of selected endogenous control genes in human breast cancer cells. Mol Cell Probes. 24(2): 87-92. PubMed Abstract 

Nishihara, M. et. al. (February 2010). Transcriptional regulation of the human establishment of cohesion 1 homolog 2 gene. Biochem Biophys Res Commun. 393(1): 111-7. PubMed Abstract

Perisic, T. et. al. (February 2010). Valproate and amitriptyline exert common and divergent influences on global and gene promoter-specific chromatin modifications in rat primary astrocytes. Neuropsychopharmacology. 35(3): 792-805. PubMed Abstract

Duthie, S. J. et. al. (January 2010). Folate deficiency alters hepatic and colon MGMT and OGG-1 DNA repair protein expression in rats but has no effect on genome-wide DNA methylation. Cancer Prev Res (Phila, Pa). 3(1): 92-100. PubMed Abstract

Majid, S. et. al. (January 2010). Genistein reverses hypermethylation and induces active histone modifications in tumor suppressor gene B-Cell translocation gene 3 in prostate cancer. Cancer. 116(1): 66-76. PubMed Abstract

Fritzenwanger, M. et. al. (2009). Cardiotrophin-1 induces tumor necrosis factor alpha synthesis in human peripheral blood mononuclear cells. Mediators Inflamm. 2009: 489802. PubMed Abstract

Fujishiro, H. et. al. (December 2009). Involvement of DNA hypermethylation in down-regulation of the zinc transporter ZIP8 in cadmium-resistant metallothionein-null cells. Toxicol Appl Pharmacol. 241(2): 195-201. PubMed Abstract

Watson, J. A. et. al. (October 2009). Generation of an epigenetic signature by chronic hypoxia in prostate cells. Human Molecular Genetics. 18(19): 3594-604. PubMed Abstract

Fujishiro, H. et. al. (August 2009). Involvement of DNA hypermethylation in down-regulation of the zinc transporter ZIP8 in cadmium-resistant metallothionein-null cells. Toxicology and Applied Pharmacology. 241(2): 195-201. PubMed Abstract

Zhang, X. Y. et. al. (June 2009). Promoter cloning and characterization of the rabbit BK channel beta1 subunit gene. Gene. 438(1-2): 33-9. PubMed Abstract

Nakamura, H. et. al. (May 2009). Role of angiotensin II type 1 receptor on retinal vascular leakage in a rat oxygen-induced retinopathy model. Ophthalmic Research. 41(4): 210-5. PubMed Abstract

Liu, Z. et. al. (May 2009). Modulation of DNA methylation by a sesquiterpene lactone parthenolide. Journal of Pharmacology and Experimental Therapeutics. 329(2): 505-14. PubMed Abstract 

Majid, S. et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4): 662-70. PubMed Abstract

Wang, X. et. al. (January 2009). RAF may induce cell proliferation through hypermethylation of tumor suppressor gene promoter in gastric epithelial cells. Cancer Science. 100(1): 117-25. Full PDF Article

Fritzenwanger, M. et. al. (May 2008). Cardiotrophin-1 induces intercellular adhesion molecule-1 expression by nuclear factor κB activation in human umbilical vein endothelial cells. Chin Med J. 121(24): 2592-2598. Full PDF Article

Roll, J. D. et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol. Cancer. 7: 15. Full PDF Article

Miyazaki, T. et. al. (October 2007). E-cadherin Gene Promoter Hypermethylation in H. pylori-Induced Enlarged Fold Gastritis. Helicobacter. 12(5): 523-531. PubMed Abstract