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Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody

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Application: ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
Application Guarantee: 6 months
Catalog No.SizePriceQty
A-4039-02525 µg $139.00 
A-4039-05050 µg $269.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Background 
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

Concentration
1 mg/ml

Description
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the trimethylated histone H3 at K27, ChIP-grade

Purification
Affinity purification

Specificity
Broad Range, Mouse, Rat, Human

Isotype
IgG

Immunogen
A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

Formulation 
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Storage
Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles.

Alternative Names
H3K27me3 antibody; H3K27m3 antibody;HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f;Histone H3/h ; Histone H3/I;Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3

Application
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100

 

Fig. 1. Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K27me3 antibody.

Fig. 2. Dot-blot analysis of all sorts of methylation peptides using H3K27me3 antibody.

Fig. 3. Immunohistochemistry of paraffin-embedded rat testis tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens).

Fig. 4.Immunohistochemistry of paraffin-embedded rat brain tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens).

Fig. 5. Immunohistochemistry of paraffin-embedded human thyroid cancer tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens).

Fig. 6. Immunohistochemistry of paraffin-embedded human rectal cancer tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens).

Fig. 7. Immunofluorescence analysis of 293T cell using H3K27me3 antibody. Blue: DAPI for nuclear staining.

Fig. 8. Chromatin immunoprecipitation analysis of various genes from 293 cell line, using Histone H3K27 Trimethyl Polyclonal Antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

User Guide & MSDS

[User Guide]*
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Product Citations

Timoshevskiy VA et. al. (June 2016). Cellular and Molecular Features of Developmentally Programmed Genome Rearrangement in a Vertebrate (Sea Lamprey: Petromyzon marinus). PLoS Genet. 12(6):e1006103.

Eid A et. al. (February 2016). Developmental lead exposure and lifespan alterations in epigenetic regulators and their correspondence to biomarkers of Alzheimer's disease Alzheimer's and Dement. :1-9.

Barroso M et. al. (October 2015). S-adenosylhomocysteine induces inflammation through NFkB: A possible role for EZH2 in endothelial cell activation. Biochim Biophys Acta. 1862(1):82-92.

Pacini L et. al. (September 2015). Down-regulation of toll-like receptor 9 expression by beta human papillomavirus type 38 and implications for cell cycle control. J Virol.

Guo X et. al. (December 2014). Regulation of histone demethylase KDM6B by hypoxia-inducible factor-2α Acta Biochim Biophys Sin (Shanghai).

Siouda M et. al. (May 2014). Epstein-Barr virus down-regulates tumor suppressor DOK1 expression. PLoS Pathog. 10(5):e1004125.

Guo X et. al. (March 2014). Nicotine induces alteration of H3K27 demethylase UTX in kidney cancer cell. Hum Exp Toxicol. 33(3):264-9.

Saidj D et. al. (November 2013). Oncoprotein E7 from beta human papillomavirus 38 induces formation of an inhibitory complex for a subset of p53-regulated promoters. J Virol. 87(22):12139-50.

Accardi R et. al. (March 2013). Epstein - Barr virus transforming protein LMP-1 alters B cells gene expression by promoting accumulation of the oncoprotein ΔNp73α. PLoS Pathog. 9(3):e1003186.

Siouda M et. al. (December 2012). Transcriptional regulation of the human tumor suppressor DOK1 by E2F1. Mol Cell Biol. 32(23):4877-90.

Shen Y et. al. (October 2012). Expression and significance of histone H3K27 demethylases in renal cell carcinoma. BMC Cancer. 12:470.

Ding YB et. al. (September 2012). 5-aza-2'-deoxycytidine leads to reduced embryo implantation and reduced expression of DNA methyltransferases and essential endometrial genes. PLoS One. 7(9):e45364.

Ramming A et. al. (May 2012). Maturation-related histone modifications in the PU.1 promoter regulate Th9-cell development. Blood. 119(20):4665-74.

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