OCLN Recombinant Monoclonal Antibody [8H8]

▲

▼

Overlay Peak curve showing HepG2 cells stained with OCLN rMAb (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of OCLN rMAb diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0. 05% DAB.

Application:	ELISA, FC, IHC
Areas of Research:	Other Primary Antibodies
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74538-050	50µl	$299.00
A74538-100	100µl	$399.00

Request A Bulk Quote

Earn up to 399 reward points for this purchase!

Order now & get it by Tuesday, December 30th

Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. (Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes.

Description
OCLN Recombinant Monoclonal Antibody [8H8]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
Q16625

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human OCLN

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Occludin, OCLN

Application
ELISA, IHC, FC; Recommended dilution: IHC:1:50-1:200, FC:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

Product FAQ

No FAQs found at this time

Browse By Category

Products

Antibodies

Primary Antibodies

Cell Structure & Function Antibodies

Chromatin & Transcription Antibodies

Histone Modification Antibodies

Signal Transduction Antibodies

Metabolism Antibodies

Immunology & Inflammation Antibodies

Antibody Panel Packs

Instruments

Research Kits

DNA Methylation

Chromatin & Transcription

Histone Methylation

Acetylation & Deacetylation

Sample Preparation

Other Histone Modifications

Proteins & Enzymes

DNA Methylation/Demethylation Proteins

Modified Histone Proteins

Peptides

COVID-19 Assays

Services

DNA-based NGS Services

Chromatin-based NGS Services

qPCR-based Services

ELISA-based Services

Support

Technical Support

Quality Assurance

Resources

Corporate

Company Information

Procurement

Communication

OCLN Recombinant Monoclonal Antibody [8H8]