Product Overview
Superoxide dismutase (SOD) is one of the most important enzymes in defending oxidative stress. SOD catalyses the dismutation of superoxide anion (O2-) into hydrogen peroxide and molecular oxygen (O2). Oxidative stress through reactive oxygen species (ROS) plays an important role in the etiology and progression in certain diseases such as cancer, heart disease, and various autoimmune disorders. ROS have shown to cause cellular damage, enzyme inactivation, and more particularly, epigenetic damage of DNA. Thus, the determination of SOD activity is necessary in various research fields related to human diseases. There are several methods used for measuring SOD activity, yet most of these methods have some drawbacks in selectivity, rapidity, and convenience. The EpiQuik Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric) addresses these problems by using a unique procedure to measure SOD activity. The kit has the following features:
- The fastest procedure available, which can be finished within 60 minutes.
- Very simple -- just one step.
- High throughput microplate format with consistent reaction conditions.
Product Details
Inhibition Curve Prepared Using SOD from Bovine Liver. The kit utilizes a water-soluble tetrazolium salt that produces a water-soluble formazan dye upon reduction with a superoxide anion (6). The rate of the reduction with O2- is linearly related to the xanthine oxidase (XO) activity and is inhibited by SOD. Therefore, the IC 50 (50% inhibition activity of SOD or SOD-like materials) can be determined by this colorimetric method.
Product Components
SD1 (Dilution Buffer)
SD2 (10X SOD Assay Buffer)
SD3 (SOD Substrate)
SD4 (SOD Indicator Solution)
SD5 (Reaction Enzyme, 0.1 U/ml)
SD6 (SOD Standard, 10 U/µl)
96-Well Microplate
User Guide
Home » DNA Damage & Repair » EpiQuik Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric) 
