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EpiQuik Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric)

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Citations (10) | Write a Review
Inhibition Curve Prepared Using SOD from Bovine Liver. The kit utilizes a water-soluble tetrazolium salt that produces a water-soluble formazan dye upon reduction with a superoxide anion (6). The rate of the reduction with O2- is linearly related to the xanthine oxidase (XO) activity and is inhibited by SOD. Therefore, the IC 50 (50% inhibition activity of SOD or SOD-like materials) can be determined by this colorimetric method.
Input Type: Cell Extracts
Research Area: DNA Damage & Repair
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
OP-0001-196 assays $95.00 
OP-0001-22x96 assays $167.00 
OP-0001-35x96 assays $299.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

Superoxide dismutase (SOD) is one of the most important enzymes in defending oxidative stress. SOD catalyses the dismutation of superoxide anion (O2-) into hydrogen peroxide and molecular oxygen (O2). Oxidative stress through reactive oxygen species (ROS) plays an important role in the etiology and progression in certain diseases such as cancer, heart disease, and various autoimmune disorders. ROS have shown to cause cellular damage, enzyme inactivation, and more particularly, epigenetic damage of DNA. Thus, the determination of SOD activity is necessary in various research fields related to human diseases. There are several methods used for measuring SOD activity, yet most of these methods have some drawbacks in selectivity, rapidity, and convenience. The EpiQuik Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric) addresses these problems by using a unique procedure to measure SOD activity. The kit has the following advantages:

  • The fastest procedure available, which can be finished within 60 minutes.
  • Very simple -- just one step.
  • High throughput microplate format with consistent reaction conditions.

Principle & Procedure
The EpiQuik™ Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric) utilizes a dye that produces a water-soluble formazan upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by measuring superoxide anion-caused formation of water-soluble dye (color intensity). The higher the SOD activity, the less the formation of formazan and the lower the OD value.

Product Components

SD1 (Dilution Buffer)
SD2 (10X SOD Assay Buffer)
SD3 (SOD Substrate)
SD4 (SOD Indicator Solution)
SD5 (Reaction Enzyme, 0.1 U/ml)
SD6 (SOD Standard, 10 U/µl)
96-Well Microplate
User Guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Calzetta L et. al. (April 2018). N-Acetylcysteine protects human bronchi by modulating the release of neurokinin A in an ex vivo model of COPD exacerbation. Biomed Pharmacother. 103:1-8.

Qian Y et. al. (March 2018). Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury. Cell Physiol Biochem. 45(6):2268-2282.

Ashwini P. Aithal et al. et. al. (October 2017). Therapeutic Potential of Human Bone Marrow Derived Mesenchymal Stromal Cells in Combination with Silymarin against Carbon Tetrachloride Induced Liver Cirrhosis in Wistar Rats JKIMSU. 6(4)

Diao Z et. al. (February 2017). Increased renal oxidative stress in salt-sensitive human GRK4γ486 V transgenic mice. Free Radic Biol Med.

Naghii M et. al. (June 2015). Antioxidants therapy: An alternative for androgen deprivation therapy (ADT) to decrease prostate-specific antigen (PSA) level Integr Cancer Sci Ther.

Gupta N et. al. (April 2015). Fasudil and SOD packaged in peptide-studded-liposomes: Properties, pharmacokinetics and ex-vivo targeting to isolated perfused rat lungs. Int J Pharm. 488(1-2):33-43.

Naghii M et. al. (November 2014). The efficacy of antioxidant therapy against oxidative stress and androgen rise in ethylene glycol induced nephrolithiasis in Wistar rats. Hum Exp Toxicol.

Risitano A et. al. (June 2012). Platelets and platelet-like particles mediate intercellular RNA transfer. Blood. 119(26):6288-95.

Martin HL et. al. (June 2012). Pharmacological manipulation of peroxisome proliferator-activated receptor γ (PPARγ) reveals a role for anti-oxidant protection in a model of Parkinson's disease. Exp Neurol. 235(2):528-38.

Liao YJ et. al. (April 2010). Deficiency of glycine N-methyltransferase results in deterioration of cellular defense to stress in mouse liver. Proteomics Clin Appl. 4(4):394-406.

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