The EpiQuik™ In Situ Histone H3-K27 Tri-Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3-K27 tri-methylation in situ using cultured adherent cells.

WHY CHOOSE THE EPIQUIK™ IN SITU HISTONE H3-K27 TRI-METHYLATION ASSAY KIT?

• Quick and efficient procedure, which can be finished within 3 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
• Measurement of in situ histone H3-K27 tri-methylation without the need to prepare cell lysates.
• Microplate format makes the assay suitable for high throughput analysis of agents that increases or inhibits H3-K27 tri-methylation.
• Simple, reliable, and consistent assay conditions.

EpiQuik™ In Situ Histone H3-K27 Tri-Methylation Assay Kit is a whole cell-based detection of tri-methylated H3-K27. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The tri-methylated H3-K27 is then detected by an anti-tri-methyl H3-K27 antibody. The ratio or amount of tri-methylated H3-K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:
	MDA-231 cancer cells were grown in microplate wells for 48 h and tri-methyl histone H3-K27 was detected with this kit.

GK1 (10X Wash Buffer)
GK2 (Permeabilizing Buffer)
GK3 (Blocking Buffer)
GK4 (Antibody Buffer)
GK5 (Capture Antibody, 1000 µg/ml)*
GK6 (Detection Antibody, 200 µg/ml)*
GK7 (Developing Solution)
GK8 (Stop Solution)
30% H2O2 Solution
Tri-Methyl H3-K27 Control (20 µg/ml)
8-Well Control Strips
Microplate
User Guide

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.