The EpiQuik™ Global Pan-Methyl Histone H3-K9 Quantification Kit (Fluorometric) is a convenient package of tools that allows the experimenter to specifically measure global mono-, di-, and tri-methylation of histone H3-K9 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL PAN-METHYL HISTONE H3-K9 QUANTIFICATION KIT (FLUOROMETRIC)?

• Quick and efficient procedure, which can be finished within 3 hours.
• Innovative fluorometric assay without the need for radioactivity, electrophoresis, and chromatography.
• Simultaneous quantification of mono-, di-, and tri-methylated H3-K9 with the detection limit as low as 1 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
• The control is conveniently included for the quantification of the amount of mono-, di-, and tri-methylated H3-K9.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Global Pan-Methyl Histone H3-K9 Quantification Kit (Fluorimetric) is designed for simultaneously measuring global histone H3-K9 mono-, di-, and tri-methylation. In an assay with this kit, the methylated histone H3 at lysine 9 is captured to the strip wells coated with antibodies specifically for mono-, di-, and tri-methyl H3-K9. The captured mono-, di-, and tri-methylated histone H3-K9 can then be detected with a labeled detection antibody, followed by a fluorescent development reagent. The ratio of mono-, di-, and tri-methylated H3-K9 is proportional to the intensity of fluorescence. The absolute amount of the methylated H3-K9 can be quantitated by comparing to the standard control.

SCHEMATIC PROCEDURE:

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.