The EpiQuik™ Acetyl-Histone H3 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3 acetylation and DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Acetyl-Histone H3 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.

WHY CHOOSE THE EPIQUIK™ ACETYL-HISTONE H3 ChIP KIT?

• The fastest procedure available, which can be finished within 5 hours.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Columns for DNA purification are included: save time and reduce labor.
• Compatible with all DNA amplification-based approaches.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Acetyl-Histone H3 ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for acetyl-histone H3 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H3 antibody and a negative control normal mouse IgG. Chromatin in the cells is extracted, sheared, and added into the microwell immobilized with the antibody. Acetyl-histone H3 protein-DNA complex is captured by the antibody and DNA is released and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

SCHEMATIC PROCEDURE:

CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3A (Pre-Lysis Buffer)
CP3B (Lysis Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Protease Inhibitor Cocktail (100X)*
Proteinase K (10 mg/ml)*
Normal Mouse IgG (0.5 mg/ml)*
Anti-Acetyl-Histone H3 (1 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

Moreno, R. et. al. (March 2011). The β-interferon scaffold attachment region confers high-level transgene expression and avoids extinction by epigenetic modifications of integrated provirus in adipose tissue-derived human mesenchymal stem cells. Tissue Eng Part C Methods. 17(3): 275-87. PubMed Abstract

Li, W. et. al. (November 2010). IL-32: a host proinflammatory factor against influenza viral replication is upregulated by aberrant epigenetic modifications during influenza A virus infection. J Immunol. 185(9): 5056-65. PubMed Abstract

Moreno, R. et. al. (October 2010). The β-Interferon Scaffold Attachment Region Confers High-Level Transgene Expression and Avoids Extinction by Epigenetic Modifications of Integrated Provirus in Adipose Tissue-Derived Human Mesenchymal Stem Cells. Tissue Engineering Part C: Methods. Epub ahead of print. PubMed Abstract

Molteni, R. et. al. (March 2010). Reduced function of the serotonin transporter is associated with decreased expression of BDNF in rodents as well as in humans. Neurobiol Dis. 37(3): 747-55. PubMed Abstract