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DNA Methylation Quantification

DNA methylation plays an important role in normal organismal development and in cellular differentiation in higher organisms. Gene expression, as well as the development of nearly all types of cancer, are also tied to DNA methylation. For example, global decrease in 5-methylcytosine content (DNA hypomethylation) is likely caused by methyl-deficiency due to a variety of environmental influences, and has been proposed as a molecular marker in multiple biological processes such as cancer. Global quantification of DNA methylation is crucial for understanding the roles that gene expression and silencing play in the development of cancer and other diseases.


MethylFlash Methylated DNA Quantification Kit (Colorimetric)Cat #P-1034

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)Cat #P-1039

MethylFlash Hydroxymethylated DNA Quantification Kit (Colorimetric)Cat #P-1036

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric)Cat #P-1041

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PurposeColorimetrically measure global 5mC in DNAColorimetrically measure global 5mC in DNA directly from urineColorimetrically measure global 5hmC in DNAColorimetrically measure global 5fC in DNA
Starting MaterialDNAUrineDNADNA
Input Range50-200 ng0.5-5.0 µl50-200 ng 100-300 ng
Optimal Input Amount100 ngµl100 ng300 ng
Total Time4 hours4 hours4 hours4 hours
Detection Threshold200 pg of meDNA30 pg of meDNA40 pg of hMeDNA1 pg of fC DNA
Type of Methylation Detected5-methylcytosine5-methylcytosine5-hydroxymethylcytosine5-formylcytosine
Specificity>1000:1 meDNA:unMeDNA>1000:1 meDNA:unMeDNA>1000:1 hMeDNA:unMeDNA>1000:1 fC DNA:unMeDNA
FormatELISA-like Microplate-basedELISA-like Microplate-basedELISA-like Microplate-basedELISA-like Microplate-based
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