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BackgroundModulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
DescriptionHistone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationBuffer: PBS with 0.09% sodium azide, 50% glycerol., pH7.3.
SpecificityBroad Range, Mouse, Rat, Human
IsotypeIgG
Uniprot IDQ16695/P68431
PurificationAffinity Purified
ImmunogenA synthetic monomethylated peptide around K4 of human histone H3 (NP_003520.1)
StorageShipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles
Alternative NamesH3K4me1 antibody; H3K4m1 antibody; HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3
ApplicationWB, IHC, IF/ICC, ChIP, ChIP-seq, ELISA; Recommended dilution: WB 1:2000 - 1:10000, IHC 1:50 – 1:200, IF/ICC 1:50 - 1:200, ChIP 5μg antibody for 5μg-10μg of Chromatin, ChIP-seq 1:20 - 1:100, ELISA - recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
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A guided immunohistochemistry protocol resource that helps researchers choose antibody staining workflows for FFPE tissue, frozen tissue, fluorescent IHC, antigen retrieval, controls, and troubleshooting.
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A guided immunofluorescence and immunocytochemistry protocol resource that helps researchers optimize antibody staining for cultured cells, tissue sections, multiplex fluorescence, and phospho/PTM targets.
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A guided ELISA protocol tool that helps researchers choose the right plate-based immunoassay workflow for sandwich, indirect, direct, or competitive ELISA, with antibody setup, controls, plate layout planning, and troubleshooting guidance.
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