The EpiQuik™ In Situ Histone H3-K27 Tri-Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3-K27 tri-methylation in situ using cultured adherent cells.

WHY CHOOSE THE EPIQUIK™ IN SITU HISTONE H3-K27 TRI-METHYLATION ASSAY KIT?

• Quick and efficient, which can be finished within 3 hours.
• Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.
• Measurement of in situ histone H3-K27 tri-methylation and no need to prepare cell lysates.
• Microplate format makes the assay to be suitable for high throughput analysis of agents that increase or inhibit H3-K27 tri-methylation.
• Simple, reliable, and consistent assay conditions.

EpiQuik™ In Situ Histone H3-K27 Tri-Methylation Assay Kit is a whole cell-based detection of tri-methylated H3-K27. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The tri-methylated H3-K27 is then detected by anti-tri-methyl H3-K27 antibody. The ratio or amount of tri-methylated H3-K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:

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